Jegarubee B. Email & Phone Number

Ottawa, Ontario, Canada

Hamilton, ON Canada

• Designing and performing in vivo and in vitro experiments to investigate the new strategies for providing broad protection against acute respiratory viral pathogens such as influenza virus in mouse model
• Conducting experiments to understand the mechanism of action of memory alveolar macrophages and other cell types in lung in prior adenovirus infected mouse model following influenza infection involving flow cytometry.
• Contributed to write a review article in journal of leukocyte biology about innate immune memory of tissue resident macrophages
• Trained a visiting scholar and graduate students to perform bronchoalveolar lavage, nasal administration of substances and blood collection from abdominal artery in mouse model and to culture mammalian cells in sterile.
• Regularly participating and presenting research findings and peer-reviewed papers in immunology journal club and lab meetings

Guelph, Canada

• Performed experiments to investigate the role of chicken microbiota in induction of immunity against Marek’s disease virus (MDV) infection in chickens by administering the antibiotic cocktail, evaluating the changes in.
• Performed an experiment to investigate the role of chicken microbiota and probiotics in induction of immunity by MD vaccines against MDV infection by administering a cocktail of Lactobacillus species in ovo and orally.
• Optimized and conducted in vivo experiments which involved flow cytometry to examine the changes in the cellular profile and real-time PCR techniques to investigate and characterize the presence of regulatory.
• Contributed to others’ projects by collecting and processing samples and performing flow cytometry

Guelph, ON Canada

• To characterize innate responses induced in chicken embryos, encapsulated Toll-like receptor (TLR) 4 and 21 ligands were administered and induction of innate responses was demonstrated by RNA extraction from various.
• Various TLR ligands (TLR-Ls) were tested for their ability to inhibit MDV infection in vitro and Poly(IC) was identified as potent immunomodulatory agent which significantly reduced MDV infection
• To induce immunity against MD by TLR-Ls as standalone prophylactic agents, TLR-Ls encapsulated PLGA nanoparticles were administered to chicken embryos and post-hatch chickens and significant reduction in tumor.
• To enhance the immune responses induced by MD vaccine, the effect of in ovo administration of CpG encapsulated PLGA nanoparticles as adjuvant with MD vaccine followed by MDV infection at hatch was investigated and.
• Trained three summer students to perform laboratory techniques including sample collection, RNA extraction, plasmid extraction, cDNA synthesis and real-time PCR
• Contributed to others’ projects by helping in administering substances to animal models, sample collection and performing laboratory assays

United States

• To investigate a preventive strategy for transmission of Mannheimia haemolytica from domestic sheep to bighorn sheep, colony PCR was performed following culture of bacteria from nasal and oropharyngeal swabs collected.
• To investigate the correlation between hemolysis and cytotoxicity of Mannheimia haemolytica leukotoxin, clinical isolates of Mannheimia haemolytica was confirmed for the leukotoxin gene by colony PCR followed by.
• To maintain the continued supply of laboratory stuffs including reagents, media and appliances, regularly managed inventories and ordered them as needed
• Supervised several research assistants and mentored three summer students to perform laboratory techniques including bacterial culture, plating of samples, PCR and gel-electrophoresis
• Contributed to others’ projects in handling experimental animals, necropsy, sample collection, sample processing, data collection and reviewing manuscripts
• Routinely managed daily activities of the laboratory

United States

• For the identification and characterization of inhibition of growth of Mannheimia haemolytica by Pasteurella multocida, in vitro experiments were performed which involved bacterial culture, colony-forming unit assay.
• To investigate the ability of inhibition of Mannheimia haemolytica by Bibersteinia trehalosi in the nasopharynx of domestic sheep, Mannheimia haemolytica positive animals were colonized with Bibersteinia trehalosi by.
• To investigate the horizontal gene transfer of leukotoxin gene from Mannheimia haemolytica to Bibersteinia trehalosi, as a pilot experiment, co-cultured bacteria were screened by colony PCR for the transferred gene.

Sri Lanka

Diagnosis, treatment, prevention and control of small animal diseaseSpaying and neuteringEducated clients about small animal health care and management

Doctor Of Philosophy - Phd, Viral Immunology

PhD thesis: Induction of Immunity against Marek’s Disease using Toll-Like Receptor Ligands Recipient of Ontario Trillium Scholarship.

Master Of Science (Ms), Veterinary Infectious Diseases

Masters thesis: Inhibition of the growth of Mannheimia haemolytica by other gram negative bacteria. 2011

Bvsc, Veterinary Medicine And Animal Science