Jennifer Luker Email & Phone Number

San Diego, Ca, Us

San Diego, Ca, Us

• Redesigned vacuum control system lines and connections, reorganized the consumables via improved inventory management utilizing a kanban system, and changed ordering process by optimizing vendor connections.• Created forms and procedures for monitoring equipment location tracking with calibration status, hybridoma clone requests and updates, and cell bank generation with cryopreservation location.• Completed 3 hybridoma screening campaigns, assumed responsibility for all hybridoma downstream scale-up requests for purification, antibody sequencing prep, and cryopreservation (cell bank generation).• Performed Flow Cytometry assays to monitor antibody expression on BaF3 and CHO over-expressing cell lines, using Stratedigm S1000EON and automated high-throughput plate loader, with analysis performed using FlowJo.• Single cell sorted for 3 campaigns of hybridoma clones into approximately 70 plates with 384-wells, and 8 cell lines of over-expressing cell clones on the BD FACSAria Fusion.• Completed puromycin titration study to establish kill curves and limited growth curve, via luciferase reaction measuring ATP (CTG), based on higher than typical/standardized puromycin concentrations currently used. Performed characterization of expression levels based on titration study as well as generated antibody standard curves presented with secondary staining.• Performed IgG ELISAs for antibody confirmation in hybridoma clone serum-free supernatant.

San Diego, California, Us

• Developed a kanban system for working inventory of 200+ items, optimized lab space, and managed ordering and stocking supplies for MSAT/PD Dept general usage and planned large projects; maintained stock despite ongoing shortages utilizing multiple databases, including Smartsheet, for tracking and management. Worked with vendors and supply chain to assist with distribution of media, reagents, and consumables and was able to supply other departments with stock shortages.• Managed LN2 inventory of MSAT Dept to include: Feeder Cell Production distribution to Fate CMO, MSAT, PD, R&D, and QC; MSAT iNK production lots to be distributed to PD, MSAT, and QC; MSAT iNK experimental lots for further testing.• Completed 3 lab reorganizations, to include: 2 building moves, within the 3 years at the company. The reorganizations consisted of managing/monitoring equipment transfers, setting up the new lab spaces, and optimized the locations based on the changing stock needs, for a team of 20 people.• Developed and maintained production management reports for K562 and NK batch lot comparative analysis, consolidation, optimization, and yield projections for future lots for internal and CMO use.• Drafted and reviewed protocols and batch records for planned projects approved by multiple departments.• Performed multiple Flow cytometry assays for functionality and phenotype on K562 and NK cell lines using BD FACS CantoII or Beckman Coulter CytoFlex, with analysis performed using FlowJo.• Subject matter expert (SME) analysis review of phenotypic flow cytometric assays.• Managed NK expansion production for assay development groups testing per Fate cGMP protocols.• Successfully reduced potential for endotoxin concentration in product administration by providing data from and managing protocol led clinical administration process studies for product comparative analysis.• Trained employees on K562, OP9, and NK Fate cell culture process expansion, analysis, and flow-based assays.

San Diego, California, Us

• During COVID, realized and notified Manufacturing Department about termination of critical reagent production, allowing time for testing of alternate products before running out of stock.• Performed growth optimization testing of K562 and NK cell lines cultured in Cytiva Xuri Bioreactors in volumes of 1-10L, Wilson Wolf G-REX Vessels, and Corning spinner flasks.• Programed the Thermo Scientific CryoMed Controlled Rate Freezer to perform freezer profile load testing and optimization for AT Vials and monitored using the Thermocouple temperature monitoring control system.• Worked with a team to perform stability testing of NK Cell Lines for extended holds at various temperatures both pre and post thaw to be applied in the manufacturing department.• Performed cell irradiation using Rad Source RS 3400 Blood Irradiator and confirmed proliferation prevention via 35-day cell growth assay testing.• Performed IgG ELISAs on wash medium to test Unifuge vs LOVO wash capabilities during cell volume reduction and cleaning on K562 Cells.• Demonstrated expertise in training and performing filling and freezing Cryovials, CryoMACS bags, and Aseptic Technologies (AT) Vials using repeater pump or Bausch pump and Thermo Scientific CryoMed Controlled Rate Freezers.

San Diego, Ca, Us

It was a start-up company under the COI Pharma umbrella.• Cell culture to include: Isolating, maintaining, harvesting, counting (hemocytometer and cellometer), cryopreservation, and thawing of macrophage cells from human whole blood and mouse bone marrow through monocyte differentiation.• Constructed, revised, and followed protocols for multiple types of assays and was responsible for laboratory supply inventory, ordering, and stocking to maintain what was required.• Multi-tasked 6 different types of full panel testing plates for multi-array cytokine assays using Meso Scale Discovery QuickPlex SQ 120 or ELISA on co-cultured macrophages with bacteria.• Performed Flow Cytometry using a Novocyte by Acea for macrophage cell phenotype analysis.• Tested oxygen consumption and extracellular acidification analysis of cells using Agilent Seahorse XFp Analyzer.• Performed murine in vivo assays including IP and IM injections as well as tissue dissection and processing for downstream cell-based experiments.

• Manufacturing of reagents and protein purification following SOPs and cGMP protocols.

• Tissue culture to include: culturing, maintaining, Mycoplasma treatment, counting (using hemocytometer and Nexcelom Cellometer), cryopreservation, and thawing of Hybridoma cells.• Gel electrophoresis performed on cell lysates followed by Western Blotting (immunoblotting).• Determined isotypes of Hybridoma clones using ELISA.

San Marcos, Ca, Us

• Used an elevator pitch to the University STEM Dean to gain the opportunity to work with software, ICM-Molsoft and Crystallographic Object-Oriented Toolkit (COOT), helping solve her X-ray crystallographic data.

San Marcos, Ca, Us

• Utilized bacterial expression system with Escherichia coli, performed purification with affinity chromatography, and characterized the protein structure and protein-protein interactions using circular dichroism and fluorescence spectroscopy, to obtain and analyze Curli Csg-encoded proteins.

New York, Ny, Us

Filed paperwork and other items and created an organization system for the itemsControlled access to the facilitiesMonitored camera systemsMonitored vital equipment utilizing several software programsHandled and routed multi-line phone system for a company of 600+ employees

Bachelor’S Degree, Biochemistry

Associate Of Science (A.S.), Forensic Science And Technology