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South Korea

전략투자팀

South Korea

미국 매사추세츠 보스턴

Activation of PKR leads to the global shutdown of protein synthesis and inhibition of viralreplication, cell cycle progression, and cell growth by activating its substrate, eIF2a. ◎ Developing an activator is potent for inhibiting viral infection. I have determined the complexstructure of PKR and its inhibitor by X-ray diffraction experiment ○ Expression of recombinant proteins○ Purification of recombinant proteins○ Crystallization of protein/ small molecule… Show more Activation of PKR leads to the global shutdown of protein synthesis and inhibition of viralreplication, cell cycle progression, and cell growth by activating its substrate, eIF2a. ◎ Developing an activator is potent for inhibiting viral infection. I have determined the complexstructure of PKR and its inhibitor by X-ray diffraction experiment ○ Expression of recombinant proteins○ Purification of recombinant proteins○ Crystallization of protein/ small molecule complex, and determination of crystal structure. ○ Characterization of a small molecule under the context of PKR and eIF2a activation in vitro by various biochemical assays (thermal melting shift, EMSA, SEC-MALS, and kinase assay with a radioisotope). Show less

수원

I-motif or C4 is a four-stranded DNA structure with a protonated cytosine: cytosine base pair(C+:C) found in cytosine-rich sequences. I have found that oligodeoxynucleotides containing adenine and cytosine repeats form an I-motif-like structure which is stable at a physiological pH with magnesium, and have named this structure “adenine: cytosine i-motif (AC i-motif or AC4)”. AC i-motif contains C+:C base pairs intercalated with A+:C base pairs between protonated adenine and cytosine. By… Show more I-motif or C4 is a four-stranded DNA structure with a protonated cytosine: cytosine base pair(C+:C) found in cytosine-rich sequences. I have found that oligodeoxynucleotides containing adenine and cytosine repeats form an I-motif-like structure which is stable at a physiological pH with magnesium, and have named this structure “adenine: cytosine i-motif (AC i-motif or AC4)”. AC i-motif contains C+:C base pairs intercalated with A+:C base pairs between protonated adenine and cytosine. By investigation of the AC i-motif present in the CDKL3 promoter (AC i-motifCDKL3), one of many AC i-motif in the genome, we confirmed that AC i-motifCDKL3 has a key role in regulating CDKL3 gene expression in response to magnesium. Furthermore, genome-edited mutant cell lines, lacking the AC i-motif formation, lost this regulation effect. Our results verify that adenine-cytosine repeats commonly present in the genome can form i-motif-like structures with a new type of non-Watson-Crick base pairing and have regulatory roles in cells, expanding non-canonical DNA repertoires. Show less

Doctor Of Philosophy - Ph.D., Molecular And Cell Biology

Bachelor Of Science - Bs, Biochemistry