Protein biochemist with expertise in purifying, sizing, and analyzing function of recombinant proteins.• Extensive protein purification experience through various techniques, including ProteinA, ion exchange, GST, and IMAC• Head biochemist for the purification and characterization for the Pan Receptor Interacting Molecule (PRIM) project.• Expertise in AKTA avant system for purification and analysesLab Manager• Maintain and procure general and lab supplies• Sales and communication liaison for ABB webstore

• Designed and performed experiments to test activity of small biologics for R&D and QA purposes in efforts to support the development of chemical methods and probes to address fundamental challenges associated with studying regulation of in vivo metabolism and signaling• Analyzed chemical libraries synthesized within the lab using methods including ABPP and WST-1 for efficacy towards lung cancer and melanoma cells through inhibition of Diacylglycerol Lipase and Kinase• Maintained lung cancer and melanoma cell lines for lab use • Maintained lab notebook and analyzed data regularly for meetings with team members and PI• Wrote and presented proposals for academic and industrial purposes

• Explored the effects of aspartate aminotransferase protein point mutations, predominately Y117W, in Helicobacter pylori, and concluded that Y117 residue is not an essential residue for activity and catalysis for the protein• Performed extensive literature analysis and sequence alignments with known aspartate aminotransferases • Conducted site-directed mutagenesis through polymerase primer extension (PIPE) mutagenesis and PCR for bacterial chemical transformations• Performed various experiments, including cloning and expression of proteins, protein purification through immobilized metal affinity chromatography (IMAC), gel electrophoresis, protein dialysis and quantification, and kinetic analysis of proteins for quality and functional assessment on a weekly basis

• Collected samples and performed DNA extraction, purification, and genetic analysis to contribute in efforts to explore the genetics of adaptation, evolution, and mechanisms of gene-environment interaction.• Assisted lab technician with daily duties

• Performed experiments including miniprep, maxiprep, gel electrophoresis, ELISA, PCR/ RTPCR for QA purposes on a weekly basis to support the R&D of biological therapeutics for the treatment of immune-oncological indications• Maintained mammalian cell culture for proteins and antibodies targeted for the treatment of autoimmune disease • Designed plasmid primers and cloning vectors for mutagenesis and antibody constructs• Created a plasmid database for company and client use

• Observed and assisted researchers with experiments • Independent study on cell culture and aseptic technique• Learned and performed miniprep, maxiprep, nucleic acid and protein purification, gel electrophoresis, ELISA, PCR• Made competent cells for company use

• Observed and assisted Researchers with experiments • Independent study on cell culture and aseptic technique• Learned and performed miniprep, maxiprep, nucleic acid and protein purification, gel electrophoresis, ELISA, PCR• Made competent cells for company use