Jessica Green Email & Phone Number

San Diego, Ca, Us

Carlsbad, California, United States

https://ihopegenetichealth.org/

Carlsbad, California, United States

Product development

Sequencing R&D

San Diego, Ca

Sequencing R&D

Greater San Diego Area

-Next-generation sequencing: Load, maintain, and troubleshoot Illumina sequencing platforms for routine production and non-routine investigative purposes; platforms include Genome Analyzer (GAIIX), HiSeq2000, MiSeq, HiSeq2500, and HiSeqX-Platform-lead: As Platform-lead of over 10 molecular assays be responsible for training new analysts, troubleshooting failures, and data trending-Process improvement: Work with members in and outside of the department to streamline processes to increase efficiency and reduce turnaround time of finished goods-Presentations: Create and deliver presentations providing a description of reagent composition and chemistry, assay critical steps, and in-house metrics used to determine pass or fail of a QC event-SOP updates and corrections: Update work instructions for library preparation to reflect current lab practices and any process changes to ensure procdures are in compliance with regulated documentation

Greater San Diego Area

-Quality control testing: Characterization and stability testing of reagents used in preparing DNA, RNA, and smRNA for next-generation sequencing by routine and non-routine molecular biological assays and analytical methods-Data analysis and review: Approval and release or failure of reagents after data analysis in accordance to its adherence to set specifications-Good documentation: Use cGDP to maintain accurate paper and electronic records of in process samples to capture lot information and instruments used

Greater San Diego Area

Worked for Illumina, Inc as a Quality Control Associate

-Assay development: Design a luminescence expression assay by altering conditions (protein/siRNA concentrations, additives, incubation times, cell density, etc.) to obtain desired information on the activity of the protein/siRNA complex in question-Data analysis: Neatly compile all data collected into charts using MS Excel-Vivarium management: Order, receive, and properly house all mice, as well as check food and water levels daily and euthanize mice due to sickness and/or old age -Continue with all duties performed as an intern

-Expression assays: Conduct knockdown experiments by transducing cells with a protein/siRNA complex, isolating RNA, creating cDNA then performing qPCR -RNA isolation: Isolate and purify RNA from harvested mice tissues samples-Measure Nucleic Acid OD: Obtain concentration of DNA and RNA of purified samples using a NanoDrop-Mammalian tissue culture: Passaging cells, seeding cells, 6K to 50k per well of a 24 to 96 well plate, freezing and thawing cells, maintaining an inventory of all frozen cell lines, prepare cell line-specific media-Train: Train new interns in tissue culture and laboratory upkeep-Laboratory support: Maintaining adequate supplies of as well as ordering and restocking all consumables

-Genotyping: Extract and purify DNA from harvested mice tails, perform PCR, and genotype by gel electrophoresis-Transformations: Using a constructed plasmid, transform competent cells to plate and extract DNA from using Maxi and Mini prep procedures

-Mouse IP injections: Deliver time-sensitive IP injections of a hormone solution to 15-30 mice-Breeding: Identify female mice in estrus, set up to breed with male mice, and check plugs

-Plant flats of maize weekly -Analyze maize leaf glue impressions under a microscope to count stroma while documenting any mutations-Harvest Arabidopsis seeds from individual plants to sterilize and plate-Responsible for lab duties such as: dish washing, autoclaving, preparing broth solutions, and filing invoices

Bachelor Of Science, Biochemistry And Cell Biology

Education record

Biostatistics Bioinformatics Data Mining Introduction to Programming (Java focus)