Jesus Fernandez-Rodriguez, Phd Email & Phone Number

Paris, Île-De-France, Frankreich

• Synthetic Biology for Targeted Antimicrobials and Microbiome modulation: bacteria CRISPR-killing, base editing and function addition, DNA circuit engineering, bacteriophage engineering (modified host range, improvement of production), development of novel and innovative tools for microbiome engineering• Technology strategy analysis• Intellectual Property (IP) management, analysis and implementation• Science and Technology Communication

Paris Und Umgebung, Frankreich

• Synthetic Biology for Targeted Antimicrobials• Phagemid production and bacteriophage engineering: DNA circuit engineering, modified host range, improvement of production levels.• CRISPR-Cas9 methods: genome modifications, nuclease-mediated killing, assisted recombination, construction of genomic libraries.• Patent drafting and filing.• Advanced knowledge in debugging of complex genetic networks.• Bioprocess engineering and work pipeline design.

Großraum Boston Und Umgebung

- Synthetic Biology Center• Engineering of complex, multi-gene networks in bacteria: light-sensing circuits, genetic memory devices based on phage-integrases, proteases and optimization of protein levels to generate the desired output and production levels. • Production of industrially relevant pigments in bacteria (lycopene, indigo) by using light as an input; optimization of multi-gene pathways encoding these molecules. • Systematic debugging and troubleshooting of engineered pathways. • Advanced molecular cloning, functional screening assays of engineered circuits, flow cytometry. • Independent leading and management of three projects; supervision, training and mentoring of undergraduate students.

Vienna, Austria

Protein biochemistry and analysis of human membrane receptors: production, purification and characterization of designed nanobodies, monobodies and antibodies binding to EGF receptors; single-molecule electron microscopy.

Vienna, Austria

- Expression and purification of eukaryotic proteins in bacterial vectors.- Manipulation of C. elegans (including DNA microinjection).- General Molecular Biology techniques.

Vienna, Austria

Purification and biochemical analysis of proteins: functional human membrane receptors expressed in CHO cells; nanobodies and fluorescent proteins in bacteria; protein engineering (chimeric and truncated variants, addition of association domains, etc.) • Mammalian and bacterial cell culture. • Protein expression, purification and characterization: both in mammalian cells and bacteria; solubilization, affinity chromatography, HPLC, PAGE, crystallization, electron microscopy. • Protein biochemistry: functional protein/enzymatic assays; western-blot, ELISA, ligand-binding assays, mass-spectrometry, ultracentrifugation, microcalorimetry.

Doctor Of Philosophy (Phd), Molecular Biology

- Use of a designed coiled-coil tag as a heterodimerization tool for the three-dimensional characterization of EGF receptor dimers..

Biochemistry, Molecular Biology And Biochemistry

- MSc Biochemistry

Certificate Of Higher Education In Astronomy, Astronomy, Cosmology, Astrophysics

- Enrolled in the Certificate of Higher Education in Astronomy - Courses completed: "Introduction to Astronomy", "Introduction.