Jim Cormier Email and Phone Number

Program Support● Served as an integral member of the site-wide Leadership Team, collaborated with other team members to facilitate the AmeriCorps Summer Teaching Fellowship and Generation Teach STEAM Academy in alignment with Generation Teach’s vision, mission, and values and our shared priorities of safety, community, and growth.Classroom Observations and Teaching Fellow Coaching● Observed & met with every Teaching Fellow in my department. ● Completed observation tracker and growth conferences, including weekly teaching partnership growth conferences. Teaching Fellow Coaching and Support ● Recorded and uploaded teaching videos of each Teaching Fellow for reflection and coaching.● Previewed curriculum and supplies providing strategies and suggestions to scaffold content to support all students, including multilingual learners. ● Led department meetings and team lesson rehearsals.● Led enrichment planning and review, including giving feedback on enrichment plans.● Read and respond to department Teaching Fellows journals two times per week. GT Circle Facilitation, Student Support, and Family Communication ● Facilitated Generation Teach Circles, supported site-wide supervision, and called families.● Completed end-of-summer Teaching Fellow anecdotes and program evaluations.

Successfully instructed students in the full year core subjects of Biology, Chemistry and Biotechnology elective. Both lecture and laboratory are included and the most current Massachusetts Curriculum Frameworks followed. College preparatory and honors levels were taught successfully Tutored students after school in preparation for the Massachusetts Biology MCAS exam.Served as an after school STEM (Science Fair) coach.Assisted in the development of District level biology common assessments.Innovated, collaborated, and developed biology lessons with Universal Design for Learning (UDL) format for the intention of improvements in student learning. Collaborated in a cross-curricular team setting for student academic and emotional monitoring and development purposes.

Successfully instructed students in the full year core subject of Chemistry. Both lecture and laboratory were included and the most current Massachusetts Curriculum Frameworks were followed as a guide. Both general and honors levels were taught successfully.• Attained an $11,000 donation from ThermoFisher to alleviate an emergency equipment deficiency.• Initiated and implemented a collaboration between Leominster High School – Center for Technical Education Innovation and Fitchburg State University Science Faculty for Science Fair Project guidance.• Initiated and implemented a collaboration between Leominster High School – Center for Technical Education Innovation and Fitchburg State University for Dual Enrollment for Honors Level Chemistry Students at no cost to students.

• Spring 2017 CHEM 1000 - Chemistry in a Changing World 3 credits. 4 hours 01/05/17 - 04/18/17 Monday – Thursday 3:00 pm – 4:30 pm *This is a dual enrollment course taught at Leominster High School for LHS students only. This is a chemistry course for non-science majors only. The purpose of this course is to provide a basic knowledge of chemistry so that one can understand many of the real-life situations that have a foundation in chemistry. This will be accomplished by considering real-world situations, many of which have an environmental focus: for example, the ozone hole, global warming, energy, carbon footprint, water, acid rain, air pollution, basic organic chemistry, green chemistry and the concept of sustainability.

Instructing students of all ages and skill levels of drumming and in the styles of Rock, Jazz, Blues, Bossa nova, Samba and Reggae.

Instructing students in subjects of chemistry, biology, meteorology and physical science. The boys instructed are from the ages of 13 through 16 in a year round therapeutic environment.

Laboratory Manager/ Research Associate IIThe goal of this laboratory is to identify and characterize basic function of transcription factors and their roles in normal and abnormal roles in hematopoietic cell differentiation.Laboratory Management Responsibilities: Design, develop, implement, execute and monitor systems to allow the daily function of the lab. Systems include, purchasing, ordering, inventory, lab safety, and general housekeeping. Other duties Mycoplasma contamination testing,Maintain laboratory equipment, track inventory, order supplies, and supervise physical, chemical, radiation and biological safety. Research Associate IIMolecular BiologyResponsibilities: Basic and complex molecular cloning, oligonucleotide primer design, PCR, plasmid DNA purification, DNA sequencing analysis, DNA transfection and transduction, lentiviral clone construction and production, siRNA knockdown, RNA purification, RT-PCR and QPCR, . Cell CultureResponsibilities: Culturing and maintenance of HEK 293FT, U937, NIH3T3, HT-1080 and HL-60 cells for in-vitro experiments. Protein ChemistryResponsibilities: Western blottingFlow CytometryResponsibilities: Designed and executed in-vitro experiments analyzed via flow cytometry.

Research Assistant III/ Laboratory Manager The goal of this laboratory is to study the basic mechanisms of Chagas’ disease specifically, invasion, intracellular growth, and survival and of the parasite Trypanosoma cruzi. My duties include designing and completing various experiments in molecular biology and protein chemistry to attempt to identify the basic mechanisms of Trypanosoma cruzi invasion as well as the day to day supervision of operations in a busy laboratory.ManagementResponsibilities: Explore, test, and evaluate new technologies versus cost of supplies via interaction with sales representatives. Maintain lab equipment in good working order, track inventory, order supplies, and supervise physical, chemical, and biological safety. Train all levels of laboratory personnel molecular biology and protein chemistry techniques.SafetyResponsibilities: Attend and participate in monthly Laboratory Manager Meetings for the Immunology and Infectious Disease Department. Attend periodic Harvard University ROM (Research Operation Management) meetings. Molecular BiologyResponsibilities: Basic and complex molecular cloning, oligonucleotide primer design, PCR, knockout vector construction, plasmid DNA purification, DNA sequencing analysis, RNA purification, QPCR assay.FermentationResponsibilities: Small and large scale fermentation in shake flasks using the bacteria E. coli and or yeast Pichia pastoris for protein production of targets investigated in studying Trypanosoma cruzi. Other duties include glassware preparation, buffer and media preparation.Protein ChemistryResponsibilities: Western blotting, protein purification (IMAC), FPLC.Mycoplasma Contamination TestingResponsibilities: Responsible for the monthly testing tissue culture samples for the contamination of mycoplasma for the Burleigh laboratory

: Duties include a multitude of techniques involving molecular biology, biochemistry, protein chemistry, tissue culture as well as ordering and maintaining supplies. The goal of the use of this lab is to study the basic mechanisms of primary and secondary glialblastomas in adults in order to better understand this lethal brain cancer. Protein ChemistryResponsibilities: Protein lysate preparation, Bradford assay, and Western blotting.DNA and RNA PreparationResponsibilities: DNA and RNA preparation for microarray analysis. Molecular BiologyResponsibilities: Basic and advanced molecular cloning, PCR, QRT-PCR, RNA purification, cDNA library construction, plasmid DNA purification, Lentivirus clone construction, production and transduction, oligonucleotide primer and probe design, DNA sequencing analysis. Cell Culture Assay DevelopmentResponsibilities: LDH and ATP assays were completed for determination of viability in tumor hypoxia experiments. Tissue Culture:Responsibilities: Maintenance and manipulation of mouse and human astrocytes with various genotypes maintained for experiments used to aid in learning the basic mechanisms of glialblastoma development. Genotypes include (Ink -/- K2, Ink -/- EGFR* #12a, Ink -/- EGFR*#32, PA-8-A-Cre - EGFR*, U87, PA-4-A-Cre - EGFR*, PS-5-A-Cre-EGFR*) Mycoplasma Contamination TestingResponsibilities: Responsible for the testing tissue culture samples for the contamination of mycoplasma for the Medical Oncology department. Both PCR based testing as well as ATP assays were performed to complete testing and report timely results.

Research Associate II Molecular BiologyResponsibilities: Duties include oligonucleotide primer design, PCR, transformation of plasmid DNA into E. coli and Pichia pastoris, plasmid DNA purification, restriction enzyme analysis, DNA sequencing analysis, preparation of Pichia pastoris competent cells, glassware preparation, buffer and media preparation, ordering and maintaining of biological and chemical supplies. • Produced cytokine and chemokine protein expression clones• Produced T cell receptor positive control clones• Completed troubleshooting analysis and implemented improvements in cloning systems FermentationResponsibilities: Small and large scale fermentation in shake flasks using Pichia pastoris for cytokine and chemokine production Glassware preparation, buffer and media preparation, ordering and maintaining of biological and chemical supplies.• Streamlined shake flask production process• Implemented quality control of media and production cultures• Increased yield of in-house recombinant chemokine and cytokine productionProtein AnalysisResponsibilities: Comparative protein homology analysis, SDS-PAGE gel analysis, N-terminal sequencing sample preparation, mass spectrometry sample preparation, protein lyophilization sample preparation, HPLC, FPLC• Completed and tracked quality control testing of all outsourced proteins• A complete species - protein homology analysis was completed for each type of protein produced. This was used for bioassay development Lab Safety CoordinatorResponsibilities: Weekly - eye wash bump test and hazardous waste container inspection Monthly - eye wash and shower test, fume hood and safety cabinet review, fire extinguisher check, laboratory checklist, follow up with Associates with respect to audit deficiencies

Process Development Technician Responsibilities: Work with an interdisciplinary Technology and Development team investigating novel technical procedures and devices in order to increase efficiency in a high throughput research environment. Independently design and conduct investigations into alternative technologies in accordance with team goals.PROJECTS: Large Scale Competent E. coli Production: The purpose of this pilot project was to develop methods to produce competent E. coli cells in a large scale. The scale produced was to fulfill the needs of the Genome Center's library construction. Library Construction Characterization and Optimization: The purpose of this project is to characterize the library construction process of 6 - 8 Kb BAC libraries, increase process efficiency, reduce or eliminate chimeras, reduce cost of producing libraries while increasing quality of libraries. CpG Island Libraries: The purpose of this project is to complete a comparison of CpG island patterns between the Mouse and Human. Mouse Fosmid Multiple-Glycerol Replication Project: The purpose of this project is to replicate four copies of an original mouse fosmid glycerol via an automated process. Three of the four-glycerol replicates are to be distributed to several other institutions and eventually worldwide. The format was in 384 well plates.Jumping Libraries: The purpose of this project is to attempt to find linked genes within the Neurospora genome. These linked genes may close gaps that are needed to assemble the finished genome.

Laboratory ManagerResponsibilities: Ordering of supplies and equipment. Maintain a neat and orderly appearance of the lab. Supervise and assist employees in technical matters.Director of Safety Responsibilities: Implement and maintain biohazard, hazardous waste, and radiation safety programs according to state and federal regulations. Training of employees in an annual safety program. Specialized training to employees pertaining to their individual duties.Research AssociateResponsibilities: Transduction of bovine fetal fibroblasts with the adeno-associated viral gene knockout vector. Cell culturing of bovine fetal fibroblasts, genomic DNA extraction, and PCR screening of bovine fetal fibroblast genomic DNA for gene knockout analysis was performed.RESEARCH PROJECTS:Hematech-Kirin Collaboration: The purpose of this project is to knockout the genes of Ig antibodies in bovine via a gene-targeting vector. After the knockout is complete, the human Ig heavy chain gene will be inserted into the target region of the bovine genome. The cattle will eventually be used in human therapeutics. Hematech-University of Washington Collaboration: The purpose of this project is to obtain a higher percentage of gene targeting for Ig heavy chain gene knockout in bovine by use of an adeno-associated viral vector.

Chimeric Glucose Transporter Protein (GLUT 1): The purpose of this study is to understand the structure, function, and rate of sugar transport across the cell membrane of erythrocytes via a chimeric GLUT 1 transporter protein. My assignment was to develop a transient expression system for the purpose of attaining as much chimeric GLUT 1 protein as possible for further studies.Responsibilities: Site-directed mutagenesis was used for the purpose of engineering a unique restriction site in order to insert an HA -poly His tag for protein purification and Western Blot analysis. The excision of the GLUT 1 gene was from a pGEM3Z vector and ligated into a pcDNA3.1+ expression vector. This was done so as to transfect the latter into COS-7 cells, transiently express the chimeric GLUT 1 protein and purify by way of the poly-His tag. The purified protein was then to be placed into artificial membranes and measured for cell uptake and exit of sugars.