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Jim Vann is listed as Researcher, Consultant, Analyst at University of Wisconsin-Madison, based in Madison, Wisconsin, United States. AeroLeads shows a matched LinkedIn profile for Jim Vann.
Jim Vann previously worked as Researcher, Consultant, Analyst at Cytegen Corp. and Emeritus Scientist at University Of Wisconsin-Madison. Jim Vann holds Phd & Ms, Medical Microbiology And Immunology from University Of Wisconsin-Madison.
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Currently serving as data analyst, researcher, and consultant to CyteGen Corp. pursuing molecular mechanisms involved in the benefits of exercise.*Dedicated lab and operations manager; served one private and three university labs. *Versatile senior life scientist. *Created ~35 specialized or innovative life sciences assays. *Communicated results at 8 conferences, 9 R&D exec meetings, and in 22 publications and 17 technical reports. *Mentored 22 students. As Chief Operations Manager at Lifegen Technologies, I remained deeply involved in lab bench experimentation as well as design, proposal, and execution of complex projects. Communicated results and plans in regular conference calls and meetings with presentations to industrial R&D executives.I meet challenges to develop innovative technical methods that work. Examples: 4-primer bi-directional PCRs to solve problems genotyping insertion and deletion mutations, a nested PCR assay for reverse transcriptase activity in MS and ALS patient serum, a two-color qPCR with a dual template standard for mitochondrial DNA copy number, and a diagnostic PCR assay for Norovirus infection in mice. As a Life Sciences Lab Manager and Senior Scientist: • Proven performance organizing teams and designing experiments• Analyzing and interpreting results• Acquiring expertise in area of interest• Creating solutions, writing SOPs• Presenting proposals and results• Soft skills refined in mentoring over 30 students / employeesSpecialties:Gene expression studies; mouse diet studies; mouse models – gene knockout, transgenic, infectious disease, neurodegeneration; cell culture.Areas of expertise include:DNA microarrays (array image above: Affymetrix.com), RT-qPCR, Western blot, mitochondrial respiratory chain enzyme assays, nucleic acid and protein tissue sample preparation, primary cell isolation and culture, cytotoxicity assays, pluripotent stem cell culture and differentiation, Western Blot, cloning, genotyping, bacteriology, virology. Always eager to learn new technology and recently completed the NSF-supported Human Stem Cell Technology Certificate Program at MATC.
Listed skills include Polymerase Chain Reaction, Life Sciences, Cell Biology, Biotechnology, and 29 others.
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Madison, Wisconsin, United States
Analyze RNAseq data from mouse tissues, Somascan data from mouse plasma.Examining how well injected plasma factors from exercised mice mimic gene expression changes in actual exercise in aged mice.
Dept. Of Medical Genetics.
Prolla Lab in the Dept. of Medical Genetics.
Madison, Wisconsin
Investigated ways to increase healthy lifespan, we analyzed gene expression and functional responses to aging, calorie restriction, and diet supplementation.Designed, Organized, and managed projects and experiments including mouse diet studies.Advanced lab methods and solved problems.Designed two-color qPCR mitochondrial DNA copy number assay with standard curve.Continued laboratory research as below.Reported to parent company, presented results, proposed new projects.Managed company checking and credit card account, authorized payroll, responded to CPA.Managed the dissolution of Lifegen, December, 2017.
Madison, Wisconsin
Performed Affymetrix DNA microarrays, qRT-PCR, Western blot, mitochondrial ETS enzyme assays.Prepared protein, DNA, RNA and miRNA from mouse and RNA from human blood samples.Performed Agilent Bioanalyzer RNA quality analysis, DNA damage assay.
Dept. Of Medical Genetics, Prolla Lab., Madison, Wisconsin
Performed mouse diet studies, mouse colony management, wrote animal protocols. Bred transgenic and knockout mice. Designed bi-directional genotyping PCRs.Fulfilled the many requests from around the world for our microarray data, our mutant mouse strains and their cell lines, and for frozen tissues banked from mouse studies of aging, calorie restriction, and diet supplementation with natural compounds.Collaborated to transfer supply of our "Polg mitochondrial mutator mouse" to Jackson labs.Performed Affymetrix DNA microarrays, qRT-PCR, Western blot, Flow cytometry.Cultured MEFs, tumor cells, and performed immortalization and mouse ES cell electroporation.Performed cloning and plasmid preparation, targeting vector construction.Trained and supervised undergraduates.Responsible for radioisotope management, biosafety, and ordering supplies.After working 4 years at both UW and LifeGen, left UW to work at Dr. Prolla's company, LifeGen.
Uw Department Of Neurology; Research Service, Madison Va Hospital, Madison, Wi
Performed:Studies of neuropathogenic retrovirus-induced paralytic disease in mice.Drug treatment studies of mouse paralytic disease.Mouse colony management, wrote animal protocols, and wrote grant proposals.In vitro retrovirus infection experiments and virus preparation.Virus titers of cultures and mouse tissues. Northern & Southern blots.Cultured endothelial cells, astrocytes, and myocytes.Trained and supervised undergraduates.Responsible for radioisotope management, biosafety, and ordering supplies.Was progressing in human subjects study to measure reverse transcriptase activity in serum of ALS and MS patients and controls using nested PCR assay when lab closed due to funding problems.
Uw Dept. Veterinary Science, Will Tissue Pharamcology Lab, Madison Va Hospital
Acquired resected human lung tissue from Surgical Pathology, dissected airways, tested antagonists of airway contractility. Continued studies of endothelial cell bactericidal activity.
Una Ryan Laboratory Of Vascular Biology, Dept. Of Medicine, Miami, Fl
Studies of endothelial cell bactericidal activity. Discovered that adenosine and metabolites increase endothelial cell phagocytosis but decrease bactericidal activity. Laboratory radioisotope manager.
Activities and Societies: Badger track team, four year letter winner.
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Jim Vann works for University of Wisconsin-Madison.
Jim Vann is listed as Researcher, Consultant, Analyst at University of Wisconsin-Madison.
Jim Vann is based in Madison, Wisconsin, United States while working with University of Wisconsin-Madison.
Jim Vann has worked for Cytegen Corp., University Of Wisconsin-Madison, Lifegen Technologies, Llc, and University Of Miami.
You can use AeroLeads to view verified contact signals for Jim Vann at University of Wisconsin-Madison, including work email, phone, and LinkedIn data when available.
Jim Vann holds Phd & Ms, Medical Microbiology And Immunology from University Of Wisconsin-Madison.
Jim Vann is listed with skills including Polymerase Chain Reaction, Life Sciences, Cell Biology, Biotechnology, Western Blotting, Dna, Real Time Polymerase Chain Reaction, and Molecular Biology.
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