• Project Lead of a genome-wide CRISPR KO screen project in both high-throughput arrayed and pooled formats for improved cell therapy anti-tumor functionality.• Project Lead of a single-cell multiomic (scRNA-seq, CITE-seq, and TCR-seq) profiling project for oncology cell therapy platform deep characterization and functional biomarker identification.

• Project Lead of a single-cell multiomic (scRNA-seq, CITE-seq, and TCR-seq) profiling project for oncology immuno cell therapy platform deep characterization and functional biomarker identification.• Optimized a primary innate T cell CRISPR KI process and achieved a 3.4-fold increase in KI efficiency and a 10.9-fold increase in cell expansion compared to the previously established protocol.

• Modeled a genetic disease in organoids using CRISPR and scRNA-seq. Revealed the MoA of how dysregulations of the chromatin remodeling gene CHD7 lead to CHARGE syndrome phenotypes.• Established a CRISPR engineering and clonal cell line generation workflow from the ground up. Generated hundreds of CRISPR-engineered KO, KI, and base-edited clonal iPSC lines for disease modeling, and organoid differentiation process establishment and optimization.• Optimized the organoid differentiation process by screening 100+ culture conditions using CRISPR-engineered multiplex fluorescence reporters as readout, resulting in a 20-fold increase in target cell type generation efficiency.• Funded with an NIH R21 grant as a Principal Investigator for a pooled CRISPR activation screen proposal. Assisted my advisor with project conceptualization and grant writing on 10+ grants.

• Performed a genome-wide genetic screen in photoreceptor cells and discovered actomyosin contraction as a driving force in retinal lumen formation.• Engineered 30+ transgene variants using molecular cloning and revealed a conserved apical membrane morphogenesis mechanism in photoreceptor cells.

• Investigated the taxonomy of the tube spittlebug subfamily Machaerotinae (Hemiptera: Cercopoidea: Machaerotidae). Discovered a new genus and two new insect species.• Developed a novel method in taxonomy illustration involving automatic raster-vector image conversion in graphic softwares.