Jiwon Ryu Email & Phone Number

Bethesda, Md, Us

Differentiation of human induced pluripotent stem cells to retinal pigment epithelium cells and research on age related macular degeneration and genetics related to congenital eye disease.

Baltimore, Md, Us

•In vitro axonopathy assay system with primary animal neuron and human stem cell derived neurons •Gene editing by CRISPR technology in human embryonic stem cells •Induced pluripotent stem cell (IPS) from human fibroblast of A246E mutant PS1 patients •Developing human axotomy in vitro assay system using microfluidic chamber and differentiated human neurons •Optogenetic human stem cell/neural progenitor and functional analysis of the differentiated neuron in vitro (electrophysiology) and in vivo optogenetic analysis•Tissue clearing and 3D microscopy (Light sheet microscopy)•Investigate traumatic human brain (Motor vehicle accident, blast injury and overdose) for pathology and mechanism of traumatic brain injury•Investigate cell therapy using differentiated neural and oligodendrocyte precursors from human ES or IPS for TBI and other neurodegenerative disease •Running the laboratory, training new staff/fellows and students, assisting in the preparation of writing and editing for grant applications and publications as well as protocols, recording/reporting all laboratory activities, laboratory notes, and outcomes in a detailed and organized manner •Manage the daily operation of the Oncology Histology Core Lab which includes working closely with investigators to assess their needs and develop protocols to meet their individual needs •Review papers, grant proposals and written protocols •Monitors biohazard/radiation safety standards and proper handling of potentially hazardous chemical and biological agents and/or radiation sources•Operational activities; ordering supplies and materials (electronic billing), reviewing inventory, maintaining up to date records of tests and preventative maintenance of equipment •Regular interaction with students, vendors, post-doctoral fellows, faculty members, and other clinical fellows•Research funded by grants by Maryland Stem Cell Research Foundation (MSCRF)

Baltimore, Md, Us

• Utilize western blot analysis, IHC, ICC, silver/H&E/Cresyl Violet staining, FACS, qPCR, rtPCR, animal surgery (such as bulbotomies/brain surgery), perfusion, sectioning, confocal microscopy, karyotyping and teratoma formation assays• Investigate traumatic brain injury in human and animal models to find biomarkers for early diagnosis and imaging• Develop injury models and quantified signal staining in brain sections by stereological analysis • Research cell therapies for neurodegenerative diseases, such as Alzheimer’s, Amyotrophic lateral sclerosis, fronto-temporal degeneration and TBI• Routinely culture neural or oligodendrocyte precursors from human embryonic stem cells • Derive induced pluripotent stem cells (IPS) from Alzheimer’s disease patient fibroblasts to use as a disease model • Monitor and organized administrative documentation - IACUC and safety protocols; biohazard materials• Write, review and publish manuscripts and grant proposals• Present scientific results at national meetings

Buffalo, Ny, Us

• Investigated GLUT4 binding protein 14-3-3, GAPDH and CALM to identify roles in GLUT4 intracellular trafficking• Utilized primary rat adipocytes in biochemical and immunological analysis with membrane fractionation, western blot analysis, immunoadsorption, immunoprecipitation, MALDI-TOF-mass spectrometry, confocal and immuno-fluorescence • Extensive experience with 3T3L1 adipocytes cell culture, gene knock out/down using siRNA or shRNA, eukaryotic transfection, 2DOG uptake, CB binding assays (GLUT4 function) and membrane fusion assays • Research funded by grants by NIH, American Diabetes Association (ADA), and Veterans Administration (VA)• Wrote, reviewed and published manuscripts and grant proposals• Presented findings at national meetings

Buffalo, Ny, Us

• Investigated the role of GLUT4 binding proteins and phosphorylation in intracellular trafficking in response to insulin• Utilized primary rat adipocytes for membrane preparation by homogenization or hypotonic lysis• Analyzed by immune-adsorption/precipitation, western blot, in vitro binding and in vitro/situ phosphorylation assays• Cloned CALM genes from rat adipocytes and deposited to NCBI GenBank• Wrote and published (5) manuscripts; presented findings at national meeting as a speaker and poster presenter (8)• Research funded by grants by NIH, ADA, and VA

Buffalo, Ny, Us

• Investigated the molecular mechanism of Diabetes Mellitus and the role of GLUT4 in insulin sensitive glucose uptake• Utilized primary rat and 3T3-L1 adipocytes to prepare GLUT4 membrane fraction by differential centrifugation• Analyzed purified membrane preparation by immuno-adsorption/precipitation, western blot and in vitro binding assay• Wrote, reviewed and published manuscripts and grant proposals• Wrote and published (6) manuscripts; presented findings at national meeting as a speaker and poster presenter (5)• Research funded by grant from NIH, ADA, and VA

Seoul, Kr

• Lectured 60 undergraduate students per semester (30 students per class)• Instructed students in microbial, infectious disease and treatment principles• Prepared microbiological background information for laboratory component• Supervised laboratory performance in preparation, setup, analysis and recording results• Graded based on their class performance, reports, written test and lab project score• Utilized molecular biological, biotechnological, biochemical methods such as gene cloning, site-directed mutagenesis, protein expression and purification, northern blot, western blot, enzyme kinetics, protein modeling to investigated the structure-function relationship of alcohol dehydrogenase• Cloned triosephosphate isomerase gene from rat cDNA• Published (2) manuscripts

Seoul, Kr

• Worked for the Research Institute of Pharmaceutical Sciences – affiliate of the College of Pharmacy• Managed common equipment facilities and supervised students • Trained individuals to use DNA synthesizer, Water purification system, Spectrophotometer, HPLC, GC, IR, NMR, SEM, radioactive materials, imaging system, etc• Coordinated protocols, time sheets, log books and scheduled appropriate fees • Investigated the structure-function relationship of alcohol dehydrogenase• Utilized molecular biology, biotechnology, and biochemical methods such as gene cloning, site directed mutagenesis, protein expression and purification, northern blot, western blot, enzyme kinetics and protein modeling• Published (1) manuscript

Seoul, Kr

• Proctored Laboratory Experiment class• Coordinated weekly experimental schedules and student groups• Managed reagents and equipment for the class• Responded to students’ questions and evaluated lab performance• Taught introductory microbiological skills such as sterilization, preparation of various culture media (liquid, semisolid, and solid culture), bacterial cell culture, differential staining, selective and differential media for various pathogenic microbes, detection of contaminated microbes in water, virus infection and production, isolation of anticancer compound producing bacterial strain from natural sources (soil or water)• Investigated alcohol dehydrogenase mechanism, structure, activity, and coenzyme specificity by mutagenesis• Utilized molecular biology, biotechnology and biochemical methods such as site-directed mutagenesis, protein expression and purification, western blot, enzyme kinetics and protein modeling• Utilized mutation by UV irradiation, cloning, transformation and modification of fermentation condition• Isolated anticarcinogen producing microorganisms from natural sources to improve bioconversion• Purified and analyzed bioactive compound by TLC and HPLC chromatography; characterized and quantified by GC • Published (3) manuscripts

Phd, Pharmaceutical Microbiology And Immunology

Ms, Parmaceutical Microbiology

Bs, Pharmacology