Julia Draper Email & Phone Number

Kansas City, MO, US

San Francisco, California, United States

Reprogramming primary human CD8+ T cells as part of a project to investigate rejuvenation and aging.

Kansas City, Missouri, United States

• Introduced a luminescent reporter gene into patient-derived human induced pluripotent stem cells (iPSC) via CRISPR/Cas9 gene targeting to create new clonal cell lines
• Differentiated multiple patient-derived human iPSCs to neurons and cardiomyocytes as part of a project to investigate Vici Syndrome, a rare disorder of autophagy
• Developed a protocol for dissociating and plating differentiated cardiomyocytes and neurons onto multi-well plates for downstream assays
• Performed… Show more
• Performed fluorescent and luminescent assays to screen small molecule candidates for disease treatment in a variety of cell types, including differentiated cardiomyocytes and neurons
• Differentiated a novel, genetically-modified human iPSC line to hematopoietic progenitors as part of a collaborative project to develop an in-vitro model for infant leukemia Show less

Kansas City, Missouri Area

• Developed an optimized protocol for CRISPR/Cas9 gene targeting in human embryonic (hES) and human iPSCs via ribonucleoprotein complexes (RNPs), incorporating literature review and training from the Harvard Stem Cell.
• Successfully differentiated hES and hiPSCs to a wide variety of lineages (spinal interneuron, CNS, cardiomyocyte, hepatocyte, hematopoietic) following published protocols
• Provided recommendations on published differentiation protocols to other investigators… Show more
• Provided recommendations on published differentiation protocols to other investigators, incorporating previous differentiation experience and literature review, including cost estimates for reagents and staff time
• Derived, expanded, and cryopreserved fibroblasts from patient biopsy tissue
• Published an optimized protocol for reprogramming human fibroblasts to iPSCs via Sendai Virus

Kansas City, Kansas, United States

• Maintained human and mouse pluripotent stem cells in aseptic conditions
• Differentiated hESCs to neural lineages via embryoid bodies following established protocols
• Coordinated with other researchers to prepare differentiated neurons for injection into rat spinal cord
• Imaged cells via fluorescent microscope
• Isolated and purified DNA and RNA from tissue culture specimens
• Followed established safety practices

Master Of Science (Ms), Cellular And Molecular Biology

Ba, Greek & Roman Studies

Activities and Societies: Environmental Planning Cooperative, Multicultural Affairs