Julia Draper Email & Phone Number

Lawrence, Ks, Us

Kansas City, Mo, Us

San Francisco, California, United States

Reprogramming primary human CD8+ T cells as part of a project to investigate rejuvenation and aging.

Kansas City, Missouri, United States

● Introduced a luminescent reporter gene into patient-derived human induced pluripotent stem cells (iPSC) via CRISPR/Cas9 gene targeting to create new clonal cell lines● Differentiated multiple patient-derived human iPSCs to neurons and cardiomyocytes as part of a project to investigate Vici Syndrome, a rare disorder of autophagy● Developed a protocol for dissociating and plating differentiated cardiomyocytes and neurons onto multi-well plates for downstream assays ● Performed… Show more ● Introduced a luminescent reporter gene into patient-derived human induced pluripotent stem cells (iPSC) via CRISPR/Cas9 gene targeting to create new clonal cell lines● Differentiated multiple patient-derived human iPSCs to neurons and cardiomyocytes as part of a project to investigate Vici Syndrome, a rare disorder of autophagy● Developed a protocol for dissociating and plating differentiated cardiomyocytes and neurons onto multi-well plates for downstream assays ● Performed fluorescent and luminescent assays to screen small molecule candidates for disease treatment in a variety of cell types, including differentiated cardiomyocytes and neurons● Differentiated a novel, genetically-modified human iPSC line to hematopoietic progenitors as part of a collaborative project to develop an in-vitro model for infant leukemia Show less

Kansas City, Missouri Area

● Developed an optimized protocol for CRISPR/Cas9 gene targeting in human embryonic (hES) and human iPSCs via ribonucleoprotein complexes (RNPs), incorporating literature review and training from the Harvard Stem Cell Institute● Successfully differentiated hES and hiPSCs to a wide variety of lineages (spinal interneuron, CNS, cardiomyocyte, hepatocyte, hematopoietic) following published protocols ● Provided recommendations on published differentiation protocols to other investigators… Show more ● Developed an optimized protocol for CRISPR/Cas9 gene targeting in human embryonic (hES) and human iPSCs via ribonucleoprotein complexes (RNPs), incorporating literature review and training from the Harvard Stem Cell Institute● Successfully differentiated hES and hiPSCs to a wide variety of lineages (spinal interneuron, CNS, cardiomyocyte, hepatocyte, hematopoietic) following published protocols ● Provided recommendations on published differentiation protocols to other investigators, incorporating previous differentiation experience and literature review, including cost estimates for reagents and staff time● Derived, expanded, and cryopreserved fibroblasts from patient biopsy tissue● Published an optimized protocol for reprogramming human fibroblasts to iPSCs via Sendai Virus● Performed CRISPR/Cas9 gene editing in mouse embryonic stem cells (mESCs) and prepared cells for injection into blastocysts to create chimeric mouse models● Wrote standard operating procedures (SOPs) for stem cell maintenance, reprogramming, gene targeting, differentiation, and sterile technique that are still used today ● Created a database system for inventory management of frozen cell lines and mouse sperm bank● Distributed cell lines to other researchers in accordance with Material Transfer Agreements (MTA)● Initiated PCR-based mycoplasma testing service for quality control Show less

Kansas City, Kansas, United States

● Maintained human and mouse pluripotent stem cells in aseptic conditions ● Differentiated hESCs to neural lineages via embryoid bodies following established protocols● Coordinated with other researchers to prepare differentiated neurons for injection into rat spinal cord● Imaged cells via fluorescent microscope● Isolated and purified DNA and RNA from tissue culture specimens● Followed established safety practices● Genotyped cell lines via PCR

Master Of Science (Ms), Cellular And Molecular Biology

Ba, Greek & Roman Studies

Activities and Societies: Environmental Planning Cooperative, Multicultural Affairs