Poor food handling practices impact the lives of many children globally rendering them to high malnutrition rates, frequent illness, and poor growth and development. Each year 125,000 infants and young children die from diseases and complications attributed to foodborne illness. As the lead data scientist and statistician on this project, I am using:Pandas, Numpy, Scipy, and Statsmodels libraries to analyze responses from questionnaires provided by 424 study participantsSupervised machine learning models to predict the effect of food safety knowledge, attitudes, and practices on socioeconomic standards and trainingSupervised machine learning algorithms to identify patterns in food safety responses based on certain socio-demographic characteristicsUnsupervised machine learning clustering analyses comparing childhood and adulthood weight and diabetes clusters to identify relationships between various family households

As a postdoctoral scientist, my research was focused on how RNA splicing was involved in ancestral-related health disparities in non-small cell lung cancer (NSCLC). We aimed to identify RNA splice variants involved in cancer development by ancestry, determine the mechanism of action including the effect of modified protein molecules on downstream signaling, and lastly, target mRNA splice variants with specifically designed oligo-switching nucleotides.Used bioinformatic tools such as TCGA spliceSeq, Cansplice, CCLE, COSMIC, UCSC, and Ensmble to understand the effect of splicing on translation or the effect of CRISPR exon deletion on transcription of a target gene. Cell culture to grow NSCLC cell linesCRISPR -Cas9 technology (CRISPR vector technology) in the removal of specific exons to study the effect of RNA splicing in cancer development RT-PCR to confirm CRISPR deletionqPCR to investigate the downstream effect of exon deletion on transcription Incucyte to observe changes in cell proliferation and migrationWestern blot to observe changes in protein cell signalingPython and R programming, machine learning, and use of 5’UTR-specific bioinformatic tools to interrogate the presence of specific translation efficiency sequencesProliferation and migration phase and colorimetric( Cell-Titer Glo) plate assaysJournal article reading and preparationTraining new lab members

Data is getting created at a rapid pace. It is estimated that more than 2 quintillion bytes of data have been created each day in the last two years. As organizations experience an overflow of data, they are sparing no effort to extract meaningful insights to make smarter business decisions. In order to help you unravel the true worth of data, MIT Professional Education offers Applied Data Science Program, which aims to prepare data-driven decision makers for the future.In this program that lasts for 12 weeks, you will be able to upgrade your data analytics skills by learning the theory and practical application of supervised and unsupervised learning, time-series analysis, neural networks, recommendation engines, regression, and computer vision, to name a few.Upon successful fulfillment of requirements, you will receive a Certificate of Completion from MIT Professional Education at the end of the program.

Research Advisor: Dr. Xiaoxin Luke ChenEsophageal Squamous Cell Carcinoma (ESCC) is the sixth highest incidence cancer in the world, and has one of the highest morbidity and mortality rates among cancers. Our work involved the study of NFE2L2 or NRF2, a transcription factor that has been shown to upregulate metabolism in cancer cells, and which is commonly mutated in ESCC cases from Asia, Africa, and among African-American patients. We investigated the role of NRF2 in ethanol metabolism and esophageal carcinogenesis.In this role, I did the following:Conducted cell culture of ESCC cell lines e.g. KYSE70, KYSE180 & KYSE4103D organoid culture to test cell invasion of Nrf2−/−, wild-type, Keap1−/− mouse esophagi with or without ethanol exposure. Western blot to determine expression of NRF2, ACSS1, ACSS2, ACSS3, GAPDH and 𝛃-actin in NRF2 low and high ESCC cell lines, and Nrf2−/−, wild-type, Keap1−/− and Sox2CreER;LSL-Nrf2E79Q/+ mice. Immunohistochemistry to check the expression of NRF2 and ACSS2 human, Nrf2−/−, wild-type, Keap1−/− and Sox2CreER;LSL-Nrf2E79Q/+ mouse tissues. siRNA and plasmid transfection to determine NRF2, ACSS1, ACSS2, and ACSS3 expression Run ChIP-PCR to determine NRF2 binding to mouse Acss2 gene in Keap1−/− esophagus, but not in Nrf2−/− and wild-type esophagiRun Seahorse cell mito-stress assays to characterize the effect of NRF2, ACSS2, and ethanol on cell energy phenotype and oxidative phosphorylation in ESCCH&E staining and Immunohistochemistry Animal Handling and rodent dosing (Oral gavage and SubQ) of Nrf2−/−, wild-type, Keap1−/− and Sox2CreER; LSL-Nrf2E79Q/+ miceMicroscopy: Phase, Inverted, and Incucyte were used to view cells and quantitate cell proliferation and cell migrationImageXpress Pico microscopy was used to analyze and quantitate cell invasion of NRF2 and ACSS2 ESCC cell lines with or without ethanol exposureTraining, mentoring, and supervising undergraduate students

Responsibilities: • Assist students with various Mathematics and Science problems (Quantitative Skills Center)• Developing modules and workshops for tutor training• Mentoring and guiding student tutors• Assisting with tutor training and following of center protocols• Managing work tutor work distribution.

Responsibilities: • Provide writing, reading, and communication assistance to all WSSU students, faculty, and staff. • Assist with writing projects, speeches and oral presentations, resumes, cover letters, personal statements, and visuals for incorporation into a paper or a presentation for any course.• Assist at any point in the writing process from brainstorming through final revisions to developing an outline, and perfecting one's presentation style.• Provide skills that would help students, faculty, and staff become more confident, self-sufficient writers, readers, and communicators.

Was responsible for HIV genotype/subtype identification for samples collected from over 800 patients from Nigeria, West Africa.Research responsibilities included:RNA Isolation and purificationConfirming RNA product purification: use of NanoDropReverse Transcriptase PCRDeveloping cDNA LibraryPreparing cDNA for sequencingPresenting findings at Lab meetingsAssist in conducting Journal club meetingsTraining and mentoring undergraduate students ICH GMP training on handling viral samples obtained from human samplesCell culture training and maintenance (Malaria and Leishmaniasis)

As a part of the Bioprocessing and Research Development team at Tengion, I was trained in the manufacturing process of the Neo Kidney Augment, and Quality analysis and release testing following Standard Operating Procedures (SOPs) for Regenerative medicine products: . Neo-Urinary Conduit™, and the Neo-Kidney Augment™ (NKA). GLP/cGMP was required training for this position. Further duties included development of cell-based potency assays. Manufacturing skills used included: ● Development and maintenance of cultures ●Cryopreservation ● Maintaining cell lines and record keeping ● Tissue development (NKA selective processing methods)Skills used in Analysis and Research included:• ELISA (MMP 1-10, IL4-8)• Flow Cytometry• Metabolism potency assays• 3D and 2D Tissue Culture • Angiogenesis and Tubulogenesis assays• Real Time PCR• Gene silencingWork was conducted under Dr. Dominic Justewicz.

Managing Chemical LibrariesMeasuring chemical content for distribution

As a summer intern, and a later a Research Assistant, I developed experiment protocols for mtDNA processing prior to Next Generation sequencing.Research responsibilities included:• Identifying and enhancing a novel mtDNA extraction method• Evaluating mtDNA extraction from cells through plating and fluorescence microscopy• Conducting enzyme digestion and PCR reactions specific to human mtDNA • Electrophoresis and Restriction fragment analysis• Quantitating purity of mtDNA via Qubit fluorometer Experiments were designed and carried out independently with guidance from Dr. Guthold (Research Mentor), and Mr. Jason Gagliano (Graduate student). Research was presented at Research Day 2012, Wake Forest University

Responsibilities: • Assist students with various Mathematics and Science problems (Quantitative Skills Center).• Mentoring and guiding student tutors• Assisting with tutor training and following of center protocols

TrainingTrained in the use of the following techniques: Presto-blue Assay, MTT assay, ELISA, Flow Cytometry (Guava, Attune Acoustic, & 2100 Agilent Bioanalyzer), Angiogenesis and tubulogenesis assays, Gas Chromatography- Mass Spectrophotometry, UV spectroscopy, H-MNR, IR-Spectrophotometry, UV-Visible spectrophotometry, Bradford, Lowry, BCA, Biuretic protein assays, Size-exclusion chromatography, Ion exclusion chromatography, adsorption and absorption spectroscopy, Quantitative fluorometry (Qubit 2.0 & NanoDrop), Protein-Chromatin-DNA-mtDNA-RNA extraction, protein crystallization, Southern Blot, Western Blot, Affinity Chromatography, centrifugal chromatography, gram staining, confocal microscopy, UV microscopy, fluorescent microcopy, vector cloning, cell culture, tissue culture (tumor and immune cells), tissue dissection and homogenization (rat liver, brain, heart, and kidney), electrophoresis, PCR, Reverse-Transcriptase PCR, Real-Time PCR, bacterial transformation, GFP staining, GFP chromatography, animal surgery (rat), cardiac data analysis, animal care(rat), enzyme restriction/digestion, enzyme kinetics

I worked in the laboratory of Dr. Azeez Aileru, under Dr. Exazevia Logan. The research was focused on the effects of Ouabain on blood pressure. During experimentation phase, Ouabain was infused into the bloodstream of the normotensive and hypertensive rats left femoral vein and artery at 2Ug and 4Ug. Experimentation revealed Ouabain’s ability in triggering vasoconstriction, thus leading to hypertension. It further explained the effects of Ouabain on the hormonal system.

Patrol or monitor recreational areas such as trails, slopes, and swimming areas, on foot, in vehicles, or from towersExamine injured persons and administer first aid or cardiopulmonary resuscitation, if necessary, using training and medical supplies and equipmentInspect recreational facilities for cleanlinessExhibit their understanding of ensuring a safe work environmentFurther clarify their academic focus and career goals