John Eash Email & Phone Number

As a priority driven pharmacologist I championed in vivo studies that achieved go no-go decisions on pharmaceutical targets. By modeling metabolic dysfunction and muscle myopathy, we were able to validate targets of muscle wasting. To do so, I employed a variety of in vivo skills (compound dosing, handling, and necropsy) and was responsible to coordinate pharmacology team members to determine pharmacokinetic, pharmacodynamic, efficacy, and safety of low molecular weight compounds. I further assessed targets in these models using a variety outcomes that included insulin and glucose tolerance, body composition, muscle mass, and evoked strength. Through my ex vivo skills, I was able to generate data that helped explain efficacy results using histology, ELISA, qPCR, and multi-well plate assays. I enhanced lab productivity by training junior members to proficiently apply in-vivo and ex vivo techniques (neuromuscular function, necropsy, histology, and dosing) and by operating as the group's liaison for facility planning teams that ensured seamless function. I prepared reports and presentations were used to achieve project transitions using a variety of applications (PowerPoint, Excel, Word, GraphPad, Spotfire).

Cambridge, Ma

I was relied upon to generate protocols and design experiments to address questions relevant to drug discovery in muscle biology. In doing so, I characterized animal models of disuse atrophy (immobilization), ApcMin+ model of cancer cachexia, sarcopenia, and diet induced metabolic dysfunction to validate targets. I established assays for fibrosis and validated ex vivo biomarkers that addressed in vivo results. I developed the histology assays and analysis that is used by the disease area to determine muscle fiber size, cell type, and morphology. Automation of these assays These assays reduced week long processes to hours. I was responsible to prepare reports for manuscripts and internal documentation (PowerPoint, Excel, Word, GraphPad, Spotfire).

Cambridge, Ma

I was responsible for drafting protocols and designing experiments to answer questions pertaining to drug discovery in muscle wasting disease. I conducted studies using in-vivo techniques and skills to generate in vivo data. I established assays for voluntary activity and nerve evoked muscle strength that are used to assess muscle function. I validated study and histology endpoints from in-vivo studies that supported in vivo data. I was responsible for analyzing data for manuscripts and internal documentation (PowerPoint, Excel, Word, GraphPad, Spotfire).