- Key scientist in characterization of allosteric, competitive, slow binding, irreversible enzyme inhibitors. Established methods and assays to determine kinetic parameters.- Independently presented key data at project team meetings, proposed conclusions and paths forward- Developed biochemical and cell-based assays using AlphaLisa, Mesoscale, HTRF, Luciferase reporter gene, fluorometric and radiometric techniques.- Acquired skills in reverse phase HPLC, developed methods for separation of substrate and product for key enzymes- Established selective cytotoxicity assays for oncology targets

● Project leader of a target validation research program on TAR DNA binding protein (TDP-43). Identified novelTDP-43 inhibitors, novel protein-protein interactions, and characterized functional consequences of theseinteractions.● Published four manuscripts as 1 st author● Assay development consultant to external collaborators● Characterized novel compounds that inhibit Hsp70 ATPase activity and protein refolding activity.● Acquired skills in SPR, ITC, thermal shift, protein expression and affinity chromatography, PCR, cloning,immunofluorescence, ELISA and cell-based immunoassays. SDS-PAGE, Western blotting.

● Project co-leader for drug discovery program - held team meetings, reported progress to discovery management● Lead scientist in development of biochemical and functional assays for GPCR targets to support lead optimization● Expert in assay development problem solving and mentored other scientists● Wrote new target internal reviews, assay validation documents, and study reports to support IND filings● Key team player in implementation of ActivityBase, acquired database programming skills, provided automationand ActivityBase support● Published manuscript as 1 st author and co-authored methods chapter in Current Protocols in Pharmacology● Assisted associate scientists in assay validation, compound testing, and data quality control

● Maintained various cell lines for research personnel