John Bukowski Email & Phone Number

Lebanon

Lebanon, New Hampshire

Lebanon, Nh

• Designed, executed and analyzed experiments that improved monoclonal antibody production in a glycoengineered yeast platform• Engineered knockout and expression constructs for yeast transformation• Utilized numerous techniques including PCR, DNA sequencing, SDS-PAGE, Western Blotting and flow cytometry to characterize and select transformed yeast strains• Analyzed and compiled multiple data streams and presented findings in companywide meetings • Researched, proposed and expressed novel therapeutic proteins for cancer and auto-immune disease as part of the product development team

Greater Boston Area

• Designed, executed and analyzed experiments that improved monoclonal antibody production in a glycoengineered yeast platform• Engineered knockout and expression constructs for yeast transformation• Utilized numerous techniques including PCR, DNA sequencing, SDS-PAGE, Western Blotting and flow cytometry to characterize and select transformed yeast strains• Analyzed and compiled multiple data streams and presented findings in companywide meetings

• Managed a 3-5 member research lab that investigated early embryonic development• Utilized mammalian cell culture, luciferase assays, and electrophoretic mobility shift assays to characterize the Patched-1 promoter• Designed in situ hybridization and quantitative real time RT-PCR experiments to determine the role of alternate splice forms of the Patched-1 gene in embryonic development• Designed experiments using fluorescence microscopy to study the subcellular localization of the Hedgehog Receptor complex• Used co-immunoprecipitation and GST binding studies to identify a novel interaction between Caveolin-1 and Patched-1• Determined and characterized genetic mutations in medulloblastoma tumor DNA using SSCP and DNA sequencing• Cloned multiple genes for expression analysis in bacterial and mammalian systems• Studied protein/protein interactions using GST fusion proteins and phage expression libraries• Aided in experimental design and troubleshooting• Coordinated the operation of a 3-4 person research laboratory

• Used co-immunoprecipitation and GST binding studies to identify a novel interaction between Caveolin-1 and Patched-1• Determined and characterized genetic mutations in medulloblastoma tumor DNA using SSCP and DNA sequencing• Cloned multiple genes for expression analysis in bacterial and mammalian systems• Studied protein/protein interactions using GST fusion proteins and phage expression libraries• Aided in experimental design and troubleshooting• Coordinated the operation of a 3-4 person research laboratory

Master Of Business Administration (M.B.A.)

Bachelor Of Science (Bs), Cell/Cellular And Molecular Biology