John Buzzo Email & Phone Number

Boston, Massachusetts, United States

Boston, Massachusetts, United States

Boston, Massachusetts, United States

Columbus, Ohio Area

* Quantitative PCR* RT PCR* Developed biofilm mimetic substrates with DNA* Purification of recombinant and native DNA BII binding proteins from gram positive and gram negative bacteria* Purification of recombinant RuvABC, RusA, multiple HMGB1 variants, and nucleoid associated proteins* Fast Protein Liquid Chromatography for protein purification with Akta Pure 25* Extracellular protein isolation from multiple bacterial species* Western Blot and Dot Blot with varied membranes* In vitro acetylation modification of target proteins* TAU and Electrophoretic mobility shift assays for protein characterization* Relative protein expression quantification* Electroelution of purified proteins for rabbit anti-body production and screening* Purification of IgGs* Ferroxidase activity assays* Spectroscopic absorbance assays* Analysis of LC/MS spectroscopy data using Scaffold Software* Zeis LSM 800 Confocal Microscopy and image reconstruction of biofilms* Immunofluorescence Confocal Microscopy* Data Analysis using AxioVision, Zen Software, MatLab, ImageJ, Image Lab, GraphPad Prism8* Probiotic microsphere sample preparation for animal studies* Manuscript preparations* NIH RO1 Grant submissions* Patent disclosures* Microsoft Word and PowerPoint* Participated in meetings, presentations, and journal clubs

Columbus, Ohio Area

Molecular techniques including bacterial cloning and recombinant proteinexpression of SoxR and HCV protease* Protein expression and purification using Co-TALON and Ni-NTA gravitycolumns* HPLC purification of conjugated compounds and peptides* Manual Fmoc based peptide synthesis techniques* Inhibition and Inactivation assays of HCV protease and Anthrax LF

Portland, Oregon Area

Dr. Brad TeboGraduate Research* Molecular techniques; including plasmid integration disruption, in-framedeletion of target genes, and plasmid over expression of target genes* Developed in-vitro assays to test the effects of various cofactors, enzymesand metals on the enzymatic oxidation of Manganese (II)* Cellular fractioning and protein localization using the gram negativebacterium Pseudomonas putida GB-1* Partial protein purification using Fast Protein Liquid Chromatographyincluding hydrophobic interaction, anion and cation exchange, and sizeexclusion* Analyzing protein data from MS/MS Spectroscopy* Prepared lab research and poster presentations

Houghton, Mi

Using pH.D-7 phage display technology* Basic E.coli culture techniques* Pan, Titer and amplification of phage* Cellulase assays

Houghton, Mi

RNA, DNA, and gel extractions* Data collection and analysis* Plant tissue culture and transformations* Making various types of culture media* Extensive greenhouse work* PCR techniques* Gel electrophoresis

Porcupine Mountains Wilderness State Park- Ontonagon, Mi

Masters, Biochemistry And Molecular Biology

Activities and Societies: International Society of Microbial Ecology

Bachelor Of Science, Biological Science, Biochemistry

Activities and Societies: Mu Beta Psi National Honor Fraternity- Zeta Chapter.