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Enthusiastic Chemist with experience in laboratory techniques, quantitative analysis, and synthesis from NCSU and global teaching labs. Complimented for positivity and ability to quickly learn new tools and techniques to help complete projects faster.Selected Contributions:- Analyzed protein from marine worms to see if pH affected how quickly enzyme worked to get rid of toxin. Discovered enzyme’s heme auto-reduces if left alone for extended periods of time.- Partnered with lab that specialized in high resolution x-ray crystallography to better understand protein molecules. Mutated amino acids in protein to better understand chemistry of protein. - Helped graduate students make more DHP using bacterium plasmids. Assisted graduate student with Raman spectroscopy of DHP to gain insight on how water binds to heme in DHP. - Analyzed and collected data weekly using Excel and synthesized into graphs for easy interpretation. Solved problems with matching data to physical phenomena of protein. Commended for clean data with low error rate.- Held meetings and brainstormed unknowns and devised solutions for clearer understanding of research. Designed further experiments with graduate program students. Expertise:Atomic Absorption Spectroscopy | HPL Chromatography Combustion Analysis | Titrator | Raman Spectroscopy | Data Analysis | Excel Spreadsheets | Team Collaboration | Communication
Martech Research, Llc
View- Website:
- martechresearch.com
- Employees:
- 27
-
R And D ChemistMartech Research, LlcBishopville, Sc, Us -
R&D ChemistMartech Research, Llc Aug 2021 - PresentBishopville, South Carolina, United States-Create, conduct, and manage day-to-day experiments in the research and development lab-Run daily operations of research and development lab-Help develop new company products in the research and development lab-Analyze and report results of experiments in the research and development lab-Ensure compliance with research and development lab health and safety guidelines-Maintain lab equipment and troubleshoot or report on malfunctions -
Supervisor Of Custom CarpentryRaleigh Reclaimed Mar 2020 - PresentRaleigh, North Carolina, United States-Manage day-to-day operations including 2 employees-Run outside lumberyard with raw processing. Carefully cut trees, wood, and beams ensuring safety protocols are followed. Interface with clients to discuss design details with projects starting at $1500+ -Complete complicated, custom product designs including furniture, beams, and flooring to customer specifications-Assist with special projects assigned by President -
Undergraduate ResearchDr. Stefan Franzen’S Lab 2018 - 2020Raleigh, North Carolina, United StatesResearched enzyme kinetics of DHP protein at different pH using UV/Vis spectrometer to learn effect pH has on reaction rates and product formation. Utilized DHP’s unique enzyme properties of reacting with toxins to solve human induced environmental problems.Performed routine testing of protein with various chromatographic and spectroscopic techniques. Set up UV/Vis spectrometer, recorded information, prepared samples, and created buffer solutions at different pH levels in cuvette to determine reactions. Oxidized protein to plus 3 state. Recorded and documented data and information to analyze results, interpret methodology, and understand and solve complex theoretical problems. Cleaned tools and instruments. Quickly and accurately completed assays. Supported regulatory and safe laboratory practices.- Analyzed protein from marine worms to see if pH affected how quickly enzyme worked to get rid of toxin. Discovered enzyme’s heme auto-reduces if left alone for extended periods of time.- Partnered with lab that specialized in high resolution x-ray crystallography to better understand protein molecules. Mutated amino acids in protein to better understand chemistry of protein. - Helped graduate students make more DHP using bacterium plasmids. Assisted graduate student with Raman spectroscopy of DHP to gain insight on how water binds to heme in DHP. - Analyzed and collected data weekly using Excel and synthesized into graphs for easy interpretation. Solved problems with matching data to physical phenomena of protein. Commended for clean data with low error rate.- Held meetings and brainstormed unknowns and devised solutions for clearer understanding of research. Designed further experiments with graduate program students.
John Pupa Education Details
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Chemistry -
High School Diploma
Frequently Asked Questions about John Pupa
What company does John Pupa work for?
John Pupa works for Martech Research, Llc
What is John Pupa's role at the current company?
John Pupa's current role is R and D Chemist.
What is John Pupa's email address?
John Pupa's email address is jp****@****oft.org
What schools did John Pupa attend?
John Pupa attended North Carolina State University, Ravenscroft School.
Who are John Pupa's colleagues?
John Pupa's colleagues are Ronnie Finch, Treshawn Dixon, Paula Bare, Tieron Parks, Sean Vaughn, Nicholas Wright, Valerie York.
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