Develop next generation CAR-T immunotherapy platform technology for cancer treatment.

Biotech consulting

- Design, construct and screen recombinant antibody phage library for leukemia drug candidate development.- Protein engineering of telomerase cancer vaccines used for prostate cancer immunotherapy, western blot, gene constructions, luciferase assays, mammalian cell culture.- Identifying der(12) and der(19) chromosome translocation genomic breaking points from the lymphocytes of a deceased leukemia patient, then identifying these chromosometranslocation fusion gene partners and fusion points at cDNA level, and performing function studies of these potential oncogenes using luciferase assays, mammalian cell culture, western blot.- Developing Aspergillus fumigatus ELISA immunoassay.- Conducting quality control of cancer vaccine products for FDA regulated GMP manufacturing facility: Experimental design, writing SOPs and test records, protocol validation, performing a variety of biological product assays and operating various analytical instruments such as flow cytometry, real-time PCR, spectrophotometry, providing support for IND and other regulatory submissions.- Lab management: experiment design and execution; monitoring adherence to comply regulatory policies; budget control, overseeing supply inventories, keep lab organized; supervising lab personnel.

Stem cell assay development, study hematopoietic stem cell expansion and differentiations. Cell-based assay, gene cloning and expression, protein characterization and purification, lymphocyte purification, mammalian cell culture, cell line construction, cell and tissue immunohistochemistry staining and analysis.