Pharmaceutical Engineering Lab module instructor for bio process engineering and downstream purification. SIT Institutional Biosafety Committee memberApplied Learning Committee member

Lead and manage the development of a multi-million dollar assay kit in collaboration with a government agencyWork with key stakeholders (both internal and external) to define customer requirements, functionalspecifications for the ongoing product developmentManage ongoing communication with client regarding development milestones and technical training.Provide project presentations and hands on training at customer’s siteLead and manage said product to a successful field evaluationProvide updates and manage feedback from client regarding validation to upper managementDevelop and maintain in-house software programmes for assay kitProvide quality improvement feedbacks to customer and productManage costs in project budgetingAttended ISO13485:2016 transitional training

Gene editing of Chinese Hamster Ovary (CHO) cells using zinc-finger nuclease technology to create gene-amplifiable cell lines for biologics manufacturingGlycoengineering - generating novel CHO glycosylation mutants using CRISPR/Cas9 gene–editing technologyLecturer in the Bioprocessing Technology Internship programme at BTI

Use molecular biology techniques to construct expression vectors for recombinant protein expressionCulturing and transfecting different cell lines such as CHO, 3T3, HEK 293, HeLa for production oftherapeutic recombinant proteinGenerating CHO stable cell lines expressing recombinant erythropoietin and glucocerebrosidase using antibiotic selection and gene amplification methodsEstablish purification protocol for recombinant glucocerebrosidasePurify recombinant proteins using liquid chromatography for protein analysis such as western blot and isoelectric focusingFlow cytometry to isolate successfully gene-edited CHO cellsLab management: Schedule maintenance of lab equipment, procurement of equipment and consumables,manage cell bank and coordinate lab dutiesSet up BSL 2+ lab with equipment procurement and author associated lab standard operating procedures

Managing the installation and implementation of pre-paid electricity metering system.Singapore Power Annual Report 2003Implementation of document management system Commissioning of natural gas receiving station on Jurong Island