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@lilly.com
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John Mcelwee is listed as Senior Advisor @ Eli Lilly | PhD in Biomedical Sciences at Eli Lilly and Company, based in Indianapolis, Indiana, United States. AeroLeads shows a work email signal at lilly.com, phone signal with area code 607, 914, 845, and a matched LinkedIn profile for John Mcelwee.
John Mcelwee previously worked as Senior Advisor - Molecular Pathology and Clinical Diagnostics at Eli Lilly And Company and Research Scientist - Diagnostic and Experimental Pathology at Eli Lilly And Company. John Mcelwee holds Phd, Biomedical Sciences (Cancer Epigenetics) from Cornell University.
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Experienced research scientist with exceptional interpersonal and presentation skills, coupled with a strong background in molecular biology directly relevant to human health and disease; in particular, studying both the genetic and epigenetic regulation of cancer growth and progression using genomics-based approaches. As I approach the next step in my career, I am exploring new directions for growth, and am poised to apply my skills and expertise towards the discovery of novel drug targets.Proudly, I believe my background in academia and industry, along with cross-functional strengths in research, teaching, and writing, can make me a valuable contributor across multiple disciplines within the biotechnology and pharmaceutical industries. • Proven success and ability to excel within a multidisciplinary team/environment.• Well-established acumen to learn quickly, design, and implement project requirements, while delivering expected results within stated timelines without compromising service or quality standards.• Scrupulous regard for detail, accuracy, accountability, and unwavering follow-through.Research Skills:• Cancer Research: Target validation, breast and skin cancer models (in vitro/in vivo) • Molecular Genetics: DNA cloning, gene expression, quantitative PCR (qPCR)• Next-Generation Sequencing (NGS): Thermo Ampliseq & Illumina platforms, Agilent TapeStation• Liquid Biopsy: Exosome and CTC isolation and analysis via qPCR or NGS• Single-cell Biology: 10X Genomics Chromium X & Visium, Vizgen Merscope, and Nanostring CosMx• Cell Culture: Bacterial, mammalian (2D & 3D), and ES cell culture • Epigenetics: Chromatin immunoprecipitation and ChIP-qPCR/ChIP-seq• Transgenic Techniques: Construct design, BAC recombineering, CRISPR-Cas9• Mouse Models: Generation of xenograft and transgenic mice, mouse handling/management• Drug Validation: Cell viability/cytotoxicity assays and xenograft drug delivery (IP/SC)• Molecular Biology: DNA/RNA/Protein extraction-purification, Western/Southern/Northern blot• RNA Interference (RNAi): Stable (lentiviral shRNA) and transient (siRNA) cell line generation• Cell Biology: Immunohistochemistry (IHC) & IF, confocal microscopy, RNAscope, ELISA, flow cytometry
Listed skills include Molecular Biology, Cell Biology, Cell Culture, Genetics, and 48 others.
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Indianapolis, Indiana, Us
Leading multiple projects that apply multiomics-based tools (e.g., NGS, qPCR, spatial transcriptomics, and proteomics) to aid in developing and utilizing disease-specific biomarkers.
Indianapolis, Indiana, Us
Primary research is within the sphere of personalized medicine and tailored therapeutics, using liquid biopsy and genomics-based tools (NGS) to identify novel and actionable biomarkers to aid in the treatment of cancer.
Indianapolis, Indiana, Us
Performing liquid biopsy and tissue-based biomarker research across multiple therapeutic areas, with a primary focus on oncology drug development and tailoring.
Ithaca, Ny, Us
• Characterized mechanisms regulating the epigenetic phenomena of DNA and histone methylation at the imprinted Rasgrf1 locus in mice via genetic engineering (transgenic and knockout via traditional and CRISPR/Cas9 methods). • Integral member of a multidisciplinary research project (with Cornell Applied Physics Department) that worked towards the development and validation of assays critical for the next-generation profiling of multiple epigenetic states.• Development and optimization of chromatin assays for Single Chromatin molecule Analysis at the Nanoscale (SCAN) to address novel biological questions from epigenomics studies relevant to human reproduction, development, and disease.
Ithaca, Ny, Us
• Target validation: established novel link between the epigenetic enzyme PADI2 and the progression of breast cancer using standard molecular biology techniques in both cell-based systems and genetically modified mouse models.• Compound validation: evaluated the efficacy of a PADI2 small molecule inhibitor as a potential drug candidate in the treatment of breast cancer progression both in vitro (2D/3D cell culture) and in vivo (xenograft model of human DCIS).• Generated a novel transgenic mouse model of skin cancer, establishing that PADI2 is sufficient for the development of skin neoplasias; molecular and pathological analysis showed lesions advanced to invasive squamous cell carcinomas.• Managed and played a key role in an interdisciplinary collaboration (with Scripps Chemistry Department) during the evaluation of two generations of PADI2 small molecule inhibitors as potential cancer therapeutics.
Tarrytown, New York, Us
• Directly responsible for establishing and implementing a successful sperm cryopreservation program for the maintenance of transgenic mouse lines; organized and trained staff during the transition of program to a separate department.• Organized and facilitated the development of transgenic and knockout mice using BAC recombineering to genetically modify mice in a precise and high-throughput manner for the KnockOut Mouse Project (KOMP).• Constructed transgenic BAC-targeting vectors for ES cell-based gene targeting by homologous recombination.
Ithaca, Ny, Us
• Performed comparative genomics based research using positional cloning and molecular cell biology techniques to help the mapping and cloning of genes/mutations involved in canine retinal degeneration and their human orthologs.• Participated in the development of biosensors for select bio-warfare agents, such as Bacillus anthracis (Anthrax), and helped develop and optimize dye-entrapping liposomes for the hybridization assay used for detection.• Conducted research and helped support investigations in reproductive biology using cell biology techniques to elucidate the functions of sperm and the subsequent organization of membrane sub-domains known as lipid rafts.
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John Mcelwee works for Eli Lilly and Company.
John Mcelwee is listed as Senior Advisor @ Eli Lilly | PhD in Biomedical Sciences at Eli Lilly and Company.
AeroLeads has found 1 work email signal at @lilly.com for John Mcelwee at Eli Lilly and Company.
AeroLeads has found 4 phone signal(s) with area code 607, 914, 845 for John Mcelwee at Eli Lilly and Company.
John Mcelwee is based in Indianapolis, Indiana, United States while working with Eli Lilly and Company.
John Mcelwee has worked for Eli Lilly And Company, Cornell University, and Regeneron Pharmaceuticals.
You can use AeroLeads to view verified contact signals for John Mcelwee at Eli Lilly and Company, including work email, phone, and LinkedIn data when available.
John Mcelwee holds Phd, Biomedical Sciences (Cancer Epigenetics) from Cornell University.
John Mcelwee is listed with skills including Molecular Biology, Cell Biology, Cell Culture, Genetics, Cancer, Confocal Microscopy, Science, and Pcr.
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