Johny Ijaq, Ph.D. Email & Phone Number

Bengaluru, Ka, In

Mangaluru, Karnataka, India

Hyderabad, Telangana, India

• Metabolite profiling from different types of biological matrices (biofluids, cell lines, bacterial cultures) using GC-MS and LC-MS/MS-QTOF • Contributing to set-up of standardized protocols for sample preparation• Operating and troubleshooting chromatographic/mass spectrometry equipment• Carrying out sample analyses using the targeted and/or untargeted approach• Contributing to set-up of standardized LC-MS methods for targeted analysis• Performing data analysis using the existing bioinformatics platform (MetaboAnalyst)• Keeping an up to date written and electronic record of laboratory work• Contributing to maintenance of adequate stocks in the laboratory (consumables such as chemicals, gases, disposables)• Helping ensure the functionality of key platform apparatus (service/maintenance) by liaising with the platform staff in a timely manner

Hyderabad, Telangana, India

Hyderabad, Telangana, India

𝗧𝗶𝘁𝗹𝗲: Machine Learning Based Evaluation of Human Hypothetical Proteins and Validation of Their Expression.𝗧𝗵𝗲𝘀𝗶𝘀: My PhD work mainly focused on studying human hypothetical proteins (HPs), a group of proteins for which there is no experimental evidence of translation and functional characterization. Different bioinformatics tools and methods were used for the functional annotation of hypothetical/uncharacterized protein sequences. Different available functional annotation methods were evaluated using machine learning based models. Later, to check whether or not these sequences are translatable, proteomics based approach was used to provide the analytical basis of their identification. My study established most of these hypothetical sequences as non-coding RNAs or the products of pseudogenes rather than proteins. However, confirming the expression of few HPs at protein level made it clear that these 'hypothetical' or 'uncharacterized' proteins cannot be completely ignored.PMID: 25873935PMID: 30621574https://doi.org/10.1007/s42485-020-00030-1

Project: Identification and characterization of human hypothetical proteins from cancer cell lines using proteomics and bioinformatics approaches.• Sample processing for proteomics• SDS-PAGE• Protein profiling using LC-MS/MS method • Data acquisition using Thermo Q Exactive HF Orbitrap System• Proteome discoverer• Label free quantification

Nibmg, Kalyani, West Bengal

Project: Identification of differentially expressed genes in Staphylococcus aureus in response to antibiotic exposure.

Hyderabad, Telangana, India

Taught Biochemistry, Cell biology, and Molecular biology courses to MSc students.

Pg Diploma In Patents Law, Intellectual Property Law

Phd, Biotechnology

Master'S, Biochemistry, 7.34/10

Bachelor’S, (Biotechnology, Biochemistry, Chemistry), 88%

Intermediate (Biology, Physics, Chemistry), 90.3%