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Experienced Scientist with a demonstrated history of working in the cell & gene therapy field. Strong research professional with 11+ years of experience in cell therapy and a Masters of Science focused in Biochemistry and Molecular Biology from University of Louisville.
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Director Process DevelopmentKytopen Mar 2024 - PresentCambridge, Massachusetts, Us -
Associate Director Process DevelopmentKytopen Mar 2023 - Mar 2024Cambridge, Massachusetts, Us
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Senior Scientist - Process DevelopmentKytopen Feb 2022 - Mar 2023Cambridge, Massachusetts, Us
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ScientistKytopen Nov 2020 - Feb 2022Cambridge, Massachusetts, Us
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Scientist IBluebird Bio Oct 2020 - Nov 2020Somerville, Massachusetts, UsMedia formulation and bioreactor optimization (patent pending)Lead team on process development challenges and process improvements for the Next-Gen CAR-T process -
Senior Associate Scientist IiBluebird Bio Apr 2019 - Oct 2020Somerville, Massachusetts, UsLead team developing and optimizing cryopreservation methods for PBMCs and drug product.Drive process development on cell therapies in preparation for clinical trials and manufacturing.Evaluate next-gen technology and implement novel technologies into current processes.
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Senior Associate ScientistBluebird Bio Jul 2017 - Apr 2019Somerville, Massachusetts, UsDevelop flow cytometry panels and assays for T Cell characterization and process impacts on cellsDeveloped various analytical assays including Luminex multiplex cytokine release assays Manufacturing support & SME of CAR-T processSkills and techniques include: experimental design and execution, new technologies testing and development, analytical assay development, change controls, MSAT support, bioreactor optimization, cryopreservation optimization, primary mammalian cell culture, large scale CAR-T production, cancer cell culture, lentiviral transduction, qPCR, ELISA, Luminex, flow cytometry
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Laboratory ManagerInstitute Of Molecular Cardiology, University Of Louisville Mar 2014 - Jun 2017The lab of Prof. Joseph Moore focuses on improving cardiac mesenchymal cells to enhance cardiac cell therapy.• Genetically modified human cardiac mesenchymal cells (hCMCs) to enhance their regenerative potential in heart failure.• Investigated the role of Histone Deacetylase I (HDAC1) via lentivirus and drug-based inhibition to enhance reparative capacity of hCMCs• 1st Place poster presentation awards at Research!Louisville• Skills and techniques include: primary mammalian cell culture, cancer cell culture, histology/pathology, RT-qPCR, PCR, western blotting, transformations, cell transfections, lentiviral production, lentivirus transduction, gene overexpression and knockdown, molecular cloning, immunoprecipitation, angiogenesis assays, migration assays, differentiation assays, flow cytometry, microscopy, inventory management, training and lab safety
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Processing TechnicianRegenerex Feb 2013 - Mar 2014Louisville, Ky, UsRegenerex manufactures hematopoietic stem cell products (HSCs) that induce tolerance to kidney transplants in unmatched donor-recipient patients, allowing for patient survival without lifelong immunosuppression.• Manufactured the Facilitating Cell Platform (FCRx) technology product for clinical trial transfusion• Managed inventory of manufacturing supplies and machinery• Environmental monitoring of clean room facilities• Tested products for quality control and FDA standards• Prepared standard operating procedures (SOPs)• Developed and performed validation studies for new products and procedures• Aided in the transition to cGMP facility• Trained employees on various SOPs and roles -
Biochemistry Teaching AssistantCornell University Aug 2011 - Dec 2011Ithaca, Ny, Us• Led small group and individual discussions• Created oral quizzes to reinforce biochemistry concepts• Graded quizzes and discussed answers with students individually -
Research AssistantCornell University Jun 2011 - Aug 2011Ithaca, Ny, UsThe lab of Prof. Taryn Bauerle seeks to improve agricultural output by better understanding the root structure and pathogens.• Investigated invasive species growth by analyzing nitrogen uptake and visualized root growth using Minirhizotron cameras• Explored the role of soil pathogens in apple replant disease• Aided in study to improve wines by varying planting strategies• Acknowledged in 5 papers
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Undergraduate ResearchRutgers University May 2010 - Aug 2010New Brunswick, Nj, UsThe lab of Prof. Jorge Golowasch uses electrophysiological and cell biology approaches to study the innate repair mechanism of crustacean’s nervous system after injury.• Developed and optimized assays• Visualized the localization of proteins during regeneration stages using immunohistochemistry
John Zhao Skills
John Zhao Education Details
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University Of LouisvilleBiochemistry And Molecular Biology -
Cornell UniversityBiological Engineering
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Cornell UniversityApplied Economics And Management
Frequently Asked Questions about John Zhao
What company does John Zhao work for?
John Zhao works for Kytopen
What is John Zhao's role at the current company?
John Zhao's current role is Director, Process Development, Cell & Gene Therapy.
What is John Zhao's email address?
John Zhao's email address is jz****@****ell.edu
What schools did John Zhao attend?
John Zhao attended University Of Louisville, Cornell University, Cornell University.
What are some of John Zhao's interests?
John Zhao has interest in Table Tennis, Tennis, Experimenting And Exploring, Investing.
What skills is John Zhao known for?
John Zhao has skills like Biochemistry, Matlab, Western Blotting, Immunohistochemistry, Research, Protein Chemistry, Cell Culture, Hsc, Cardiology, Qpcr, Flow Cytometry, Elisa.
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