Managing a team of scientists and specialists to perform cutting-edge mass spectrometry (MS)-based bioanalytical services including: protein identification, therapeutics quantification, biomarker discovery, post translational modification, etc. Conducting hundreds of challenging bio-analytical projects every year for clients from Biopharmaceuticals such as Novartis, BMS; Government agencies such as NIH, FDA, Academic Organisations such as Johns Hopkins University in the world of biohealth. Offering high quality bioinformatic data analysis and bioanalysis related in-depth technical consulting.

Division of Biological Standards and Quality Control/OCBQ/CBER/FDAManaged production, calibration, and inventory of CBER toxin and antitoxin standards. Worked with other agencies and WHO in harmonization of standards and reference preparations. Performed review on microbiological aspects of BLA supplements and write memos of recommendation.Managed the Division’s product lot release testing plans in accordance with testing plan requirements. Performed lot release testing to facilitate lot release process, approval of licensure and supplements. Developed and optimized testing methods; Refined and expanded existing SOPs. Conducted investigations for deviations and NCMs.Reviewed and processed manufacturer’s lot release protocols in compliance with CFR 610.9 (General Safety), CFR 610.12 (Sterility), CFR 610.13 (Endotoxin), CFR 610.14 (Potency, Identity), and CFR 610.30 (Mycoplasma). Managed and performed qualification, preventive maintenance and calibration of equipment, and qualification of reagents used in testing. Managed and performed final container filling operation, lyophilization, Nitrogen backfill procedure, and ampule sealing in an ISO 17025 Clean-room class-7 environment. Managed and performed ISO 17025 Clean-room class-7 environmental monitoring.

Experimental Immunology Branch/National Cancer Institute (NCI)/NIHInvestigated the mechanisms of Lymphocytes cytoskeleton organization and transmigration, and the conformational activation mechanism of ERM proteins. Discovered chemokine SDF-1 induced release of ERM proteins from membrane is triggered by PLC-mediated hydrolysis of PIP2, which mechanism regulates the disassembly of ERM proteins during lymphocyte cell transmigration. J Cell Biol. 184(3):451-62Developed a sub-cellular fractionation and Mass Spectrum analysis combined approach for elucidating the major components of lymphocyte microvilli and understanding the bases of Lymphocytes cytoskeleton organization and transmigration. J Proteome Res. 7(7):2911-27.Trained fellows in the lab in general biochemical techniques and cell biological techniques, especially protein expression and purification, co-immunoprecipitation, pull-down, mass spectrometry approaches, transfection confocal microscopy.

Holland LaboratoryInvestigated the mechanism of BCR signaling and cytoskeletal reorganization.Discovered Syk-mediated Tyrosine Phosphorylation triggers the recruitment of HS1 to lipid rafts and association with BCR Signalosome Complex. JBC, 279(32): 33413-20.Identified HS1 coiled-coil region acts synergistically with the repeat domain in the modulation of the Arp2/3 complex-mediated actin polymerization; modified an existing method for large scale preparation of Arp2/3 complex from bovine brain. JBC, 280(45): 37988-94Trained fellows in the lab in general biochemical techniques and cell biological techniques; supervised research projects of summer interns.

Investigated molecular basis of plasmid segregation in bacteria and the function of P1 partition proteinsDiscovered the auto-regulation of P1 partition operon; identified parS, the P1 centromere, not only exerts a major role in activating the negative regulation of P1 partition operon in trans and cis but also in cis up-regulates the expression of parB only. Journal of Bacteriology, 184: 4857-4867