Jonathan Gans Email & Phone Number

Beachwood, Oh, Us

Cleveland, Ohio, Us

Cleveland, Oh, Us

Baltimore, Us

• Ensure smooth laboratory operation via: o Keeping all equipment functional and interceding immediately in event of operational failure o Scheduling Planned Preventative Maintenance, routine sterilizations, certifications, and calibrations• Analytical and effective problem solver• Responsible for purchasing, supply chain management, and on-site inventory• Revolutionized inventory storage creating easier access for staff and easier supplies management by operations team• Cultivate and manage relationships with vendors to ensure short lead times & best pricing• Track all laboratory spending across various teams for accounting department and senior leadership• Oversee all shipping and receiving, personally handle all pallet deliveries to confirm safe handling and delivery• Instrumental in coordinating the assembly of a pilot manufacturing lab used for pre-IND studies• Created safety SOPs and track employees’ completion and adherence to them• Implemented and sustain weekend scheduling system that ensures proper staffing for all weekends and holidays, so cells are maintained and timepoints not missed• Contact point with building management to resolve any facility issues or questions• Apply Lean and Six Sigma tenets to identify inefficiencies in the work environment and find solutions to address these• Actively seek feedback and input from staff of all levels to ensure I can help meet their needs• Utilize GxP knowledge to implement a set of best lab practices

Franklin Lakes, New Jersey, Us

• Test plant produced, liquid media, products for sterility• Train new associates on proper gowning and aseptic technique• Coordinate associate schedules for rotational coverage of the lab• Involved in Kaizen Blitz to update testing standards to match USP 35 guidelines• Perform root cause analysis investigations into any product failures• Optimized processes to reduce testing errors• Investigated alternative consumable products used in testing that can help alleviate ergonomic strain• Authored a job aid for a complex piece of equipment to allow all associates to use with ease• Generated multiple projects to help streamline product testing• Responsible for lab upkeep, including, but not limited to ordering, cleaning, and making media• Collaborate with different departments on various projects to ensure completion in the most effective and efficient manner possible

Reno, Nevada, Us

• Actively involved recruiting and personnel decisions• Maintain laboratory upkeep• Manage any interpersonal issues• Oversee and provide guidance on all projects to ensure all benchmarks are met• Serve as a subject matter expert in multiple areas on projects• Train and instruct new personnel• Develop and design projects and follow-up experiments• Study strains of Klebsiella pneumoniae isolated from the International Space Station• Establish growth curves for these bacteria• Create protocol for multi-generational studies• Generate microgravity experimental conditions• Observe how microgravity and minimal inhibitory concentrations of antiseptic used aboard the ISS affect the genome and phenome of these strains over several generations• Authored a three-year NASA proposal

Baltimore, Md, Us

• Isolate bacterial RNAs for RNAseq and qPCR for bacterial pathogenesis gene expression analysis• Generate genetic constructs and recombinant bacterial strains• Create translational reporter fusions of Pseudomonas aeruginosa for study of genes controlling iron homeostasis• Establish growth curves of various Pseudomonas aeruginosa gene-knockouts under varying iron conditions to determine which genes are most dependent and controlled by iron presence• Extract RNA and created cDNA from samples to determine relative quantities of iron regulating genes under diverse growth conditions.• Develop and test a novel protocol for purifying RNA-protein complexes• Design and conduct an experimental model looking to improve antibiotic treatment of Pseudomonas aeruginosa and Staphylococcus aureus co-infection in patients suffering from Cystic Fibrosis• Optimize Staphylococcus aureus and Pseudomonas aeruginosa co-culture biofilm formation MBEC assay• Compile and analyze data, and generate relevant figures for presentations and publications• Perform bioinformatic analysis of Pseudomonas aeruginosa transcriptome and proteome to discern novel gene regulation.• Discovered novel iron regulation of sRNAs• Maintain laboratory general upkeep• Contribute to manuscript preparation via literature search, writing, and editing• Instruct and train new personnel

21201, Md, Us

• Conduct experiments on mice with various pulmonary fibrotic models, as a means of studying pathogenesis of Idiopathic Pulmonary Fibrosis (IPF)• Create new experimental protocols utilizing new murine injury techniques that give a more representative pulmonary fibrotic model of IPF• Isolate protein from experimental samples and quantify relative concentrations through Western Blot analysis• Extract mRNA from tissue samples with Trizol and create cDNA• Proficient at qPCR assay and analysis• Maintained the animal colonies: euthanized mice we would not use to prevent overcrowding in the colony, set up and maintained breeding cages to keep our desired strains active, tailed mice for genotyping, and managed breeding crosses in order to generate new strains for use in our experiments• Utilized conventional PCR to accurately genotype several strains and identify target mice from genetic crosses

Baltimore, Md, Us

• Conducted experiments with a mouse model of acute lung injury (ALI) resolution, as a means of studying the pathogenesis of Acute Respiratory Deficiency Syndrome• Performed various mouse surgeries, including: intratracheal injections via intubations, pharyngeal injections, intraperitoneal injections, retro-orbital injections, and terminal harvests• Harvested bronchoalveolar fluid, lungs, spleen, and bone marrow from mice• Determined extent of murine injury via visible phenotypes of lungs, cell counts, cytospins, protein concentration, and flow cytometry• Stained cell populations (lung, bronchoalveolar fluid, and spleen) with specific markers to determine cell populations at sites of injury, specifically looking at recruitment and maturation of macrophages and presence of regulatory T cells to determine their roles in ALI resolution• Cultured bone marrow cells in vitro and matured them into different types of macrophages (M0, M1, and M2) to then reintroduce these cells via adoptive transfer (retro-orbital injection) and observed how adopted macrophages aid in injury resolution• Colonized and maintained bacterial stocks of Pseudomonas aeruginosa and Streptococcus pneumoniae for use in a live model of ALI• Maintained the animal colonies: euthanized mice we would not use to prevent overcrowding in the colony, set up and maintained breeding cages to keep our desired strains active, tailed mice for genotyping, and managed breeding crosses in order to generate new strains for use in our experiments• Responsible for lab upkeep and maintaining supplies in the lab

Rockville, Maryland, Us

• Mutated specific nucleotides in parainfluenza virus to create an attenuated strain suitable for vaccine development• Made use of a bacterial vector to grow the viral genome and provide an environment to introduce base substitutions via specific primers• Isolated plasmids containing viral genomic information using mini and maxi preps• Ran plasmids through gel electrophoresis to determine which mutants to sequence based on fragment size• Performed both small scale and large scale PCRs to determine integrity of viral genome o Small scale performed on plasmids isolated from the mini-prep to determine if desired mutation has occurred o Large scale was performed on plasmids isolated from the maxi prep, on mutants with the confirmed mutation, to determine if the no other mutations occurred in the genome• Cultured and passaged MK2 cells for use in in vitro experiments

Blackfriars, London, Gb

Tutored students at the University of Maryland in Biochem 1. Delivered course content in an efficacious manner. Instructed pupils in useful study habits and memory tricks for the course.

Generated mutants aseptically to discover genes responsible for motility in S. marcescens, E. coli, and B. subtilis. Collected and analyzed data to determine significant results. Performed laboratory upkeep; including bench and overall lab cleanliness, taking stock of reagents, ordering those that are lacking, making various medias for the lab. Instructing and assisting new student research assistants in the lab.

Managed multiple microbiology student laboratories. Instructed graduate level teacher assistants in proper protocol and procedure for each laboratory session. Generated demonstration cultures for each experiment, so students could observe the quality of their work by comparing their results to the expected results.

Led lab lectures and graded student papers. Provided students with individualized support to work towards developing confidence and ability in microbiology.

Edison, New Jersey, Us

Observed as an Emergency Room doctor treated and diagnosed patients. Became familiar with various emergency room procedures.

Toronto, Ontario, Ca

Ensured that all campers made it to and from all activities safely. Dealt with campers' issues one on one. Provided a fun, warm, and safe environment for the kids to enjoy sleep-away camp.

Assisted patients on emergency calls; providing them both physical and emotional support. Advised patients on best course of action regarding seeking further treatment. Transported critical patients to the Emergency Room. Saved lives.

Ensured that all campers made it to and from all activities safely. Dealt with campers' issues one on one. Acted as counselor on duty at night, ensuring campers were behaving, and going to sleep. Provided a fun, warm, and safe environment for the kids to enjoy sleep-away camp.