Jonathan R. M. Millet Email and Phone Number

PhD in the Genome Regulation and Evolution group (INSERM U869 RNA : Natural and Artificial Regulation) under the supervision of Denis Dupuy, PhD.Abstract : We have developped a dual-fluorescent reporter-based system in order to follow alternative splicing event regulation in vivo. It has been applied to five different genes in the model organism Caenorhabditis elegans. Among the genes followed, two follow a potentially stochastic scheme, one show no visible sign of alternative splicing. The last display tissue specific splicing patterns but developed a toxic effect in the animal when expressed from a multicopy extrachromosomal array. To remediate this problem, we decided to develop a method that allows for simpler single copy insertion of fluorescent reporter using CRISPR-Cas technology.Funded by the Ministère de la Recherche et de l'Enseignement Supérieur Français.Conferences et seminars :19th C.elegans International Meeting (Poster) - UCLA (California, USA)Chemistry Meets Biology (Oral presentation) - IECB internal meeting (Pessac, France)Young Scientist Symposium IECB 8th edition (Oral presentation) - IECB (Pessac, France)VerMidi 18th edition (Poster) - Institut Jacques Monod (Paris, France)

Collaboration with José Eduardo Gomez, PhD (on going).

Participation in the iGEM competition (International Genetically Engineered Machine Competition).Founding member of the iGEM Bordeaux team.Project : " The Dairy Planet "Abstract : Our iGEM Bordeaux team 2013 has worked on the development of new lactic ferments able to produce flavours on their own and resveratrol also, a molecule which is considered to be responsible of good effects of red wine. This project will allow on the long run an easier production of flavoured yogurts with beneficial effects on the organisms and medical properties without using chemical substances. On top of that yogurt can be produced in an industrial way or on a small scale , the project can be used on one of those two scales.

Participation in the iGEM competition (International Genetically Engineered Machine Competition).Founding member of the iGEM Bordeaux team.Project : " The bacterial eyespot "Abstract : Our project aimed at reproducing multicellular-like behavior in the bacteria Escherichia coli. The idea came from the patterns visible on some animals, like the “pseudo-eyes” on a butterfly wing. As well, we thought about the formation of stripes on tigers or zebra. Such patterns are created thanks to a very precise genetic regulation. So what we want to do here is modeling a regulation mechanism existing in eukaryotes in a simple organism. The bacteria will share the same DNA but should be able to display a different phenotype depending on the signal received by its neighbors. The phenotype here will be the expression of three different colors. The bacteria should be able to communicate between them. A light signal is used as the first inducer of our mechanism. Then, the first cells activated by the input signal send a signal to its neighbor that will react to it by changing its color, and sending another message to the next cell. This will allow us to draw different patterns on the petri dish.Conferences et seminars :iGEM 2012 European Regional Final (Poster) - VU (Amsterdam, Netherlands)iGEM 2012 European Regional Final (Oral presentation) - VU (Amsterdam, Netherlands)

Master 2 mandatory internship in the Genome Regulation and Evolution group (INSERM U869 - RNA : Natural and Artificial Regulation) under the supervision of Denis Dupuy, PhD.Abstract : We used fluorescents reporter to follow spatio-temporal post-transcriptional 3'UTR-based regulation of several genes in C.elegans.

Master 1 mandatory internship in INSERM U1035 (Biothérapies des maladies génétiques et du cancer) under the supervision of Jean-Max Pasquet, PhD.Abstract : Nilotinib-resistant CML cells present an overexpression of Axl. We asked wether this expression could be due to a specific demethylation in cell culture using sequencing following bisulfite conversion.Conference CML 2011 Bordeaux (Participation) - Institut Bergonié (Bordeaux, France).

Non-mandatory Internship in UMR-CNRS5164 (Composantes Innées de la Réponse Immunitaire et de la Différenciation) under the supervision of Karine Massé, PhD.

Non-mandatory internship in the Lipid Research Group, under the supervision of Fatiha Tabet, PhD.