Jonathan Kwan, M.S. Email & Phone Number

Cambridge, MA, US

Cambridge, Massachusetts, US

• Manufactured novel non-viral gene therapy particles for use in mouse and rabbit models to secure Series A funding for $60MM
• Utilized Malvern ZetaSizer Ultra to measure particle concentration, hydrodynamic radius, zeta potential, and polydispersity by DLS to characterize different particle constructs and its effect on transduction, and.
• Developed a TFF concentration method using hollow fiber filter modules to scale from 1 mL preparation size up to 100 mL, and implemented a sanitization method for sterility and reusability to save process development.
• Performed cohort of quality control assays such as ELISA Endotoxin to enable batch release, supporting in vitro and in vivo models
• Identified key CMC process development milestones to align with company finances and critical path programs, and presented strategy updates to C-Suite and investor level executives
• Analyzed peptide synthesis chemistry to attribute physiochemical properties on structure and activity relationship in vitro and in vivo

North Chicago, Illinois, US

• Supported late phase monoclonal antibody in GMP production suites as a downstream SME, and designed future studies for viral clearance, process characterization, and scale-down models
• Mitigated a novel impurity species associated with a bispecific antibody through off platform depth filtration and ion exchange chromatography to keep low molecular weight species within FDA control limits
• Calculated process volume, flow rates, and raw materials needed for technical transfer to 200 L, 500 L, and 1,000 L manufacturing scale to inform technical team on process performance and to generate clinical drug.
• Examined initial facility fit of a late phase monoclonal antibody through platform purification development resulting in high-quality drug substance within FDA specification, support for drug product development team.
• Developed high-throughput small scale purification methods using TECAN to support cell culture process characterization
• Led 5S initiative by creating a team of entry-level and experienced scientists to create an inclusive environment where all lab members are responsible and accountable for workspace organization, cleanliness, and.

North Chicago, Illinois, US

• Developed process trending book for commercial stage therapeutic to retrieve historical averages of data and to support upcoming Process Performance Qualification (PPQ)
• Quantified A280 protein concentrations of commercial drug process intermediates using SoloVPE and Lunatic for technical reports
• Prepared Manufacturing Support and Technology team for PPQ by designing in-process sampling schedule for analytical testing
• Authorized data in LIMS for CPV of commercial therapeutic to identify run rule violations
• Measured primary harvest turbidity and particle size distribution to verify processed protein characteristics are within expectations
• Examined product stability of in-process intermediates of commercial drug in various time points and temperatures, and compiled results into the campaign summary report

Binghamton, NY, US

Master's Project: Skin-Interface Glucose Sensor- Sponsored and funded $106,861 by Brazilian start-up company SE7E Digital Agency, working under Dr. Ahyeon Koh in her lab: Intimately Bio-integrated Biosensors- Compile a variety of literature reviews using mind maps that involve non-invasive amperometric biosensors to optimize potential design configuration.

Binghamton, NY, US

• Critique engineering student resume and cover letters to effectively convey professional, project, and leadership experiences, and teach interviewing and networking skills to optimizing personal branding to impress.
• Fabricate captivating flyers and posters for employer recruitment events using Canva, increasing student awareness and attendance

New York, New York, US

• Designed interactive visualizations in TIBCO Spotfire as a proof of concept tool to analyze STATISTICA graphs in stability monitoring
• Interviewed multi-disciplinary engineering subject matters experts on key skills required in their discipline in the biopharma industry for the Engineering Skills Assessment, a tool colleagues utilize for individual.
• Deliberated with internal team members as well as external vendors on the Engineering Skills Assessment for robotic process automation, maximizing patient benefit by allowing colleagues to focus on higher priority work
• Implemented a survey using SurveyMonkey assessing impact and site progress, gathering feedback from a Global Engineering initiative to attract, develop, and retain Pfizer engineers and scientists

West Mifflin, PA, US

• Calibrated an InSpector 1000 portable gamma-ray spectrometers compliant with Navy regulations to support radiochemistry by reducing cost and manpower in sample collections for radiological controls
• Developed a procedure to quantify radioactivity and identify unknown radionuclides using gamma spectroscopy simplifying logistics, increasing overall efficiency, and allowing for a vast array of sample configurations
• Verified applications of NaI(TI) and LaBr detector probes for radioactive isotopic identification

West Mifflin, PA, US

• Streamlined site-integration scheduling by merging IBM TRIRIGA and Oracle Primavera P6 Professional field data
• Improved projection metrics for site-maintenance planning by creating lists, libraries, and pages in MS SharePoint
• Projected resource capabilities over three years for major industrial maintenance and repair of a nuclear prototype

• Facilitated a smooth and organized textbook packing system for student pick up
• Assisted customers in locating and selecting merchandise
• Contributed to expedite bookstore functionary; categorization and classification.
• Regulated student orders for textbook processing

Member of the start up team for the app TruNeed

Olean, NY, US

• Presented high-quality line of cutlery and culinary items to customers through in-home demonstrations
• Established new clientele through referral and lead generation
• Refined communication skills and polished personal sales techniques

• Served hot food for customer
• Performed service evaluation and discussed vital weaknesses to managers to improve efficiency in store
• Maintained a clean and safe work environment compliant to NYS regulation

Master Of Science - Ms, Biomedical Engineering

Bachelor Of Science - Bs, Cum Laude, Biomedical Engineering, Minor In Sustainability Engineering

Education record