Jonathan Joyce Email & Phone Number

Roanoke, VA, US

Blacksburg, Virginia, United States

• Investigated the contribution of neuropilin-1 (NRP-1) to SARS-CoV-2 neuronal entry in sensory neurons.
• Assessed the neurotropism of SARS-CoV-2 following intranasal infection in hACE2 transgenic mice, non-carrier mice, and guinea pigs.
• Determined the replication potential of SARS-CoV-2 in primary neuronal cultures from central and peripheral nervous system tissues from hACE2 transgenic mice and non-carrier mice.
• Quantified the recovery of infectious SARS-CoV-2 in common alcoholic and non-alcoholic beverages to assess risk for person-to-person transmission.
• Assisted in research to determine the viability of SARS-CoV-2 in/on food and beverages to better guide infection mitigation and environmental testing.
• Lead preclinical research to determine the safety, neurotropism, and serological response of a live-attenuated HSV-2 vaccine candidate after intradermal inoculation in a guinea pig model.

• Lead preclinical research to determine the safety, neurotropism, and serological response of a live-attenuated HSV-2 vaccine candidate after intradermal inoculation in a mouse model.
• Assessed the impact of inoculation route (ocular, intradermal, vaginal) on survival, neurotropism, and serological response in a mouse model to HSV-2 (MS, ΔR) versus a live-attenuated HSV-2 vaccine candidate.
• Determined clinical progression, neurotropism, and serological response in a mouse model to HSV-2 (MS) and multiple live-attenuated HSV-2 (MS) vaccine candidates after intradermal or vaginal inoculation.
• Assessed clinical progression, neurotropism, and serological response in a guinea pig model to HSV-2 (MS, ΔR) and HSV-1 (17+) after vaginal infection.
• Determined tissue tropism of Zika virus (ZIKV) in guinea pigs after subcutaneous or vaginal infection to establish a clinically relevant small animal model.
• Assisted in research to determine the tissue tropism of various adeno associated virus serotypes in guinea pigs after ocular, intradermal, intravenous, intravaginal, and intranasal inoculation.

Christiansburg, VA

• Developed training materials to train scientists in the Republic of Georgia on the creation and submission of scientific abstracts, posters, and manuscript.
• Coordinated the development of a CLSI compliant validation plan for the validation of a human anti-Brucella antibody specific fluorescence polarization assay in the Republic of Georgia.
• Collaborated with a dynamic team in the development of standard operating procedures for the development, optimization, and validation of an antigen capture ELISA for Brucella abortus and Francisella tularensis in the.
• Participated in the multi-agency development of standard operating procedures for: culturing Coxiella burnetii in axenic media, as well as the detection of C. burnetii by conventional end-point PCR, RT-PCR, and.
• Assisted in the development of public health outreach to prevent infection with African swine fever virus in high-risk groups in the Republic of Georgia.

Blacksburg, Va.

• Assisted in the development of research projects in the Republic of Georgia funded through the Defense Threat Reduction Agency's (Department of Defense) Cooperative Biological Engagement Program.
• Coordinated the compilation, analysis, and reporting of monthly, quarterly, and final project reports, project specific standard operating procedures, conference calls, minutes, and trainings of the above funded.
• Collaborated with a multi-disciplinary team in manuscript publication from data generated from the above funded projects.
• Reviewed, edited, and submitted abstracts, posters, and oral presentations from data generated from the above funded projects for international scientific conferences.
• Created a database to provide novel “home brew” assay component development for molecular assays (e.g., PCR, RT-PCR) for scientists in scientifically developing countries to detect Select Agents.
• Generated literature reviews of specific especially dangerous pathogens/Select Agents (e.g., Bacillus anthracis, Francisella tularensis, Yersinia pestis, and Crimean-Congo hemorrhagic fever virus).

Blacksburg, Va.

• Conducted molecular cloning using supplied vector DNA, de novo synthesized DNA/annealed oligos, and/or PCR products as insert through both manual techniques (gel excision, vacuum/column purification, hand ligation) and.
• Consulted and troubleshot with the Process Development Group.
• Collaborated with the Executive Assistant on the production process and implementation of several novel ideas to streamline production, enhance transparency, and increase documentation to reduce overall error rates.

Radford, VA

• Conducted research to identify the receptor used by the La Crosse virus (LACV) upon entry into the midguts of vector mosquitoes.
• Identified bacteria present in the midgut of LACV vector mosquitoes using standard microbiological and molecular techniques (e.g., 16S rRNA PCR).
• Quantified inhibitory interactions using PRNT between LACV and the previously identified midgut bacteria on the infectivity of LACV in cell culture.
• Collaborated in the development and submission of a manuscript detailing these research efforts.

Doctor Of Philosophy - Phd, Translational Biology, Medicine, & Health

Master Of Public Health - Mph, Infectious Disease

Master Of Science - Ms, Microbiology & Cell Science /Medical Microbiology

Bachelor Of Science (B.S.), Biology, General

Activities and Societies: Radford University EMS