My project investigated the role of the Nance-Horan Syndrome (NHS) protein family in fast endophilin-mediated endocytosis and breast cancer cell migration. I employed a combination of molecular biology, bioinformatic and biochemical techniques to characterise novel binding partners of the proteins NHSL1 and NHSL2.I generated NHSL1 knock-out breast cancer cell lines using CRISPR-Cas9 genome editing.I used these cells in different microscopy-based functional assays to assess the role of NHSL1 in endocytosis and breast cancer cell migration.I supervised undergraduate lab members, providing training in experimental design and laboratory techniques.

I assisted lecturers in laboratory-based practical sessions and workshops for undergraduate students.I also independently ran biochemistry tutorials for first-year medical students.

I undertook an 8-week research project in the Evans lab, focusing on characterising the interaction of N2-src with Sec23A and its effect on COPII transport. During this studentship, I was trained in a variety of cellular and molecular biology techniques including: Cell culture & transfection, immunocytochemistry, western blotting and gene cloning. I attended the 2018 North of England Cell Biology Forum in Huddersfield with members of the lab.