Jonathan Lo Email & Phone Number

Lab experience with the following techniques and skills:• Sterile cell culture• Isolation and storage of PBMCs from peripheral blood• Performing ELISA assays and analysing the subsequent data• Isolation of specific cells from tissues including tissue digestion and using MACS bead separation.• Performing intracellular cytokine staining• Design, execution and analysis of immune phenotyping and function using flow cytometry• Mouse handling and implementing in vivo experiments including IP injections• Had some experience with qPCR• Had some experience with Western BlotOther Professional Skills and courses attended• Certified Home Office Animal Licence (2012)• Certified Proactive Gas Safety cryogenic gas user in the lab (2012)• Trained and Certified Human Tissue Act trainee (2012)• Trained and Certified Good Clinical Practice trainee (2012)• Proficient at using Graphpad and FlowJo analysis• Proficient in Microsoft Excel, Word and PowerPoint

Listed skills include Pbmc Isolation, Bacterial Culture, Pcr, Primary Cell Isolation, and 10 others.