•Contracted to Grail to employ NGS analysis and molecular pathology techniques to process clinical trial and clinical patient samples for their Galleri cancer screening tool•Competently performed all experimental modules that constituted the Galleri cancer screening tool including: plasma isolation from whole blood, DNA extraction and quantification, bisulfite DNA conversion and methylation, genomic library prep, library consolidation and enrichment, qPCR, and NGS •Utilized Hamilton STAR Liquid Handler to aid in experiment execution and troubleshot minor instrument/software issues that arose•Quantified nucleic acids at various time points using Labcyte Echo and Biotek Synergy Neo2 plate reader •Performed pre-analytical and analytical procedures, inclining testing, quality control, preparation, and aliquoting of reagents, analyses, and maintaining records of tests according to CLIA/QC•Identified and documented all corrective actions taken when test systems deviated from the established performance specifications •Joined the Noncompliance Report team to tackle the documentation, resolution, and sample outcome due to operator and software-based noncompliance activities•Utilized LIMS system to track sample status and aid in experimental workflow

Performed Luciferase and GranToxiLuc-based assays in a BSL2* laboratory to measure antibody-dependent cellmediatedcytotoxicity (ADCC) responses to HIV-1 and SARS-COV2 human and non-human primate vaccinerecipients• Utilized flow cytometry in data collection of respective ADCC based assays and p24 staining.• Infected/cultured CEM.NKR.CCR5 cells with HIV-1 IMCs for ADCC based assays• Cultured SupT1 cells that were utilized as a positive control for p24 expression• Transfected both adherent and suspended cells to be used in ADCC based assays• Isolated natural killer cells from infected leukopaks to be used in SARS-COV2 degranulation assays• Performed mycoplasma testing on newly thawed cell lines• Bridged HIV-1 infected leukopaks for Luciferase/ GTL assay result reproducibility within studies• Monitored GFP expression of cells via fluorescence microscopy• Completed projects (HVTN, CAVD, PAVEG) in accordance with SOP GCLP guidelines and safety protocols• Utilized Flow Jo software to analyze flow cytometry data and Prism software to compare/compile assay results• Reviewed and authored assay-based SOPs

- Requested all samples for HVTN testing through the PTP system. Receive store and distribute all HVTN samples to all testing laboratories. Return all samples to Freezer Pro at the completion of testing.- Shipped, received, and distributed all participant samples for Duke CFAR-GAP according to IATA regulations.- Aliquoted samples and distribute between various labs- Shipped, received, stored, and distributed all Leukopak samples for the Ferrari Lab studies according to IATA regulations.- Stored, distributed for testing, and shipped, all participant samples for DIPC studies according to IATA regulations- Maintained databases and chain of custody for serum and PBMCs received from HVTN, DIPC, IQA, and samples as requested by SORF Principle Investigators.- Maintained sample requisitions and provided complete documentation for sample chain of custody for all projects (including, but not limited to, Trinidad, Duke Acute, HVTN, DIPC, and Duke CFAR samples) as part of Specimen Core.- Maintained IATA-certified LN2 Dry Shippers and Data Loggers.- Recorded temperatures/PM of all GCLP compliant equipment related to the Specimen Core repository, and designated research freezers when necessary. Monitored Minus80 daily for adequate freezer temperatures.- Responded to any freezer alarms, as required.- Reviewed any specimen-related SOPs on a bi-annual basis and provided/suggested updates or revisions.- Attended weekly Lab meetings- Attended monthly HVTN management meetings

- Contracted employee to IQVIA for work on a phase 3 clinical study aimed at determining the safety and efficacy of monoclonal antibody therapy for post-exposure prophylaxis of SARS-CoV-2 in adults.

- Prepared various biological media and buffers - Inoculated bacteria under aseptic conditions- Conducted a wide array of diagnostic tests to identify cell morphology of prokaryotes- Supervised students during open labs and assisted when needed

- Documented medical exam findings, history of present illnesses, diagnoses, and prescriptions. - Informed physicians when lab results had been received and if they were abnormal.- Prioritized and analyzed variables in high-stress situations. - Multitasked comfortably between 10 or more patients at a time.