Using pseudo-typed virus particle assays to measure neutralising antibody responses against SARS-CoV-2. Developing virus like particle assays to measure protein interactions of SARS-CoV-2.Development of assays to quantify host and viral disease markers using a novel digital PCR platform. Collaboration with industrial start-up (Blink-DX, Germany) developing a digital PCR platform using bead based nanoreactors.Technical skills: Programming languages: R | Operating systems: Linux |… Show more Using pseudo-typed virus particle assays to measure neutralising antibody responses against SARS-CoV-2. Developing virus like particle assays to measure protein interactions of SARS-CoV-2.Development of assays to quantify host and viral disease markers using a novel digital PCR platform. Collaboration with industrial start-up (Blink-DX, Germany) developing a digital PCR platform using bead based nanoreactors.Technical skills: Programming languages: R | Operating systems: Linux | Next-generation sequencing data processing | Data analysis: RNAseq, | Data interpretation and visualisation | Molecular laboratory techniques: PCR, qPCR, dPCR, ELSIA, western blot, ONT sequencing, DNA and RNA extraction. | Cell biology techniques: Cell culture of primary cells and continuous cell lines, PBMC isolation, neutralisation assay, flow cytometry, live virus culture, plaque reduction neutralisation tests (PRNTs), tissue culture infectious dose (TCID50), transfection, production of pseudo-typed virus particles. | Training and experience in containment level three facilities.Soft skills: Communication | Problem solving | Project management | Motivation | Curiosity | Teamwork | High adaptability | Flexibility Show less

Researching HIV-1 persistence and the dynamics of the latent reservoir in the context of therapeutic vaccination. Project aims to develop HIV-1 DNA and RNA quantification assays to measure ultra-low HIV-1 copy numbers from clinical samples. Techniques:- HIV-1 infection assays- Category III containment trained- Tissue culture- qPCR- PCR- Molecular biology- Bioinformatics- MinION RNA sequencing- RNA Extraction

Developing and optimizing the production of pseudo-typed virus particles with Flavivirus (Zika, Dengue) envelopes. Aim to use these particles to develop a neutralization assay for Flaviviruses. Techniques:- Molecular biology- Tissue culture- Pseudovirus production

Studying the molecular genetics of HPV and investigating markers for disease outcome, including integration of the viral genome into the host genome in high risk HPV sub-types (HPV16/18). Techniques:- Molecular biology- Tissue Culture- Pyrosequencing