Jose Vasquez Email and Phone Number

1. Upstream support in late-stage vaccine production• Aid in the increase of harvest titer within a 6-month timeline. Includes pH shift, seeding densities, and transient transfection studies• Present data to the team about any trends and effects on titer based on the changes of the parameters• Plan and led the scale-up confirmation runs up to the 50L scale for the late-stage program which includes training colleagues responsible for the engineering run at Bern site• Support in the technology transfer to an external contract manufacturing and development organization which includes PIP of engineering run, support in the writing of process description and memorandums2. Support early-stage process development• Supported with the execution feeding strategy, media screening and clarification studies at the ambr250 scale• Led the effort of translating the process from 50L stir tank reactor to 25L wave reactor for material generation purposes• Presented data to the team about the early development3. Technology development• Developed a scale down model at the 10L scale for a commercial project that involved production at the 200L scale utilizing microcarriers• Communicated with vendor possibilities of changing the design of the 10L single use bag to match the characteristics of the custom 200L single use bag

1. Supported with the completion of failure mode and effective analysis of GMP suite• Introduced new material to be used for pipeline processes• Communicated with vendors about changes in material or product• Executed a study to determine the stability of L-glutamine after 30 days of formulated medium• Exposure to adherent cell culture from T-flasks to 200L suspension bioreactor with microcarriers2. Cross trained with process development• Support in the DoE studies of a late-stage vaccine production• Exposure in suspension cell culture from shake flask and ambr250 scales to 50L scale