Joseph Rapley Email and Phone Number
Joseph Rapley's Location
Boston, Massachusetts, United States, United States
Joseph Rapley's Contact Details
Joseph Rapley work email
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Joseph Rapley personal email
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Joseph Rapley phone numbers
About Joseph Rapley
Highly experienced in the processes of manufacturing immunodiagnostic reagents, scientific research and assay development. Please see the experience section for an in-depth breakdown of achievements, knowledge and skill sets
Joseph Rapley's Current Company Details
Joseph Rapley Work Experience Details
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Technical Operations ManagerSiemens Healthineers Apr 2024 - PresentForchheim, De
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Senior BiochemistSiemens Healthineers Jun 2020 - Apr 2024Forchheim, DeI am currently involved in manufacturing reagent troubleshooting, process development of chromatography scale up procedures to increase throughput by design and process change implementation. Duties also include raw material qualification for use in manufacturing builds as well as authoring and reviewing Temporary Manufacturing Deviations (TMD), Quality Notifications (QN), feasibility/verification/evaluation reports and miscellaneous compliance documentation.I am also training and mentoring new hires as well as junior team members who can work independently as well as assist me with my projects. Previously led the Technical Operations team in the support of our new COVID immunoassays during the pandemic. The project involved supporting Manufacturing, Technology Transfer and Global Assay Development in the co-ordination of reagent builds while new procedures were implemented. Additionally I worked closely with Quality Control for testing and subsequent product release of these brand new assays.
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Biochemist 2Siemens Healthineers Feb 2016 - May 2020Forchheim, DeMy principal work revolved around troubleshooting, feasibility testing and purification of biological raw materials which are used in our biochemical diagnostic assays. Assay development has also been an integral component of my work to assist in optimizing procedures to ensure top quality materials were provided for our product builds. Assays included, infectious diseases, endogenous proteins as well as small molecules
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Senior ScientistMinerva Biotechnologies Jun 2014 - Aug 2015Waltham, Ma, UsPerformed research using immunotherapeutic techniques to inhibit cancer cell growth and metastasis by the use of therapeutic antibodies, Chimeric Antigen Receptor T-cell technology (CAR-T) and purification of growth factors. -
Assistant In BiochemistryMassachusetts General Hospital May 2011 - Jun 2014Boston, Ma, UsPerformed research in the field of molecular signal transduction and cell cycle regulation. Our work focused on mitotic regulation by the NIMA kinases and regulation of the mTOR pathway. We identified that Nek6 regulates the essential mitotic motor protein Eg5 and that this phenomenon is required for cell division. We discovered that the RNA binding protein IMP2 is a substrate for the mTOR kinase and consequently became the first reported evidence of mTOR regulating CAP independent protein translation. IMP2 is implicated in type-two diabetes and highlights a further mechanism by which mTOR may impact human disease. We also analyzed the mechanism which controls substrate binding to mTOR and determined that enhancement of substrate binding does not control mTOR signaling. We also identified that Calmodulin dependant protein kinase II (CAMKII) is capable of phosphorylating CARMA1 and that this procedure is required for T-cell receptor signalling to NFκB.My final project focused on new mechanisms of how amino acids and insulin regulate mTOR signalling and how these phenomena contribute to disease phenotypes. Duties also included supervising and training undergraduate students as well as preparation of scientific manuscripts. -
Postdoctoral FellowMassachusetts General Hospital May 2004 - Apr 2011Boston, Ma, UsSee Assistant in Biochemistry for details
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Instructor In MedicineHarvard Medical School May 2011 - Jun 2014Boston, Ma, UsPerformed research in the field of molecular signal transduction and cell cycle regulation. Our work focused on mitotic regulation by the NIMA kinases and regulation of the mTOR pathway. We identified that Nek6 regulates the essential mitotic motor protein Eg5 and that this phenomenon is required for cell division. We discovered that the RNA binding protein IMP2 is a substrate for the mTOR kinase and consequently became the first reported evidence of mTOR regulating CAP independent protein translation. IMP2 is implicated in type-two diabetes and highlights a further mechanism by which mTOR may impact human disease. We also analyzed the mechanism which controls substrate binding to mTOR and determined that enhancement of substrate binding does not control mTOR signaling. We also identified that Calmodulin dependant protein kinase II (CAMKII) is capable of phosphorylating CARMA1 and that this procedure is required for T-cell receptor signalling to NFκB.My final project focused on new mechanisms of how amino acids and insulin regulate mTOR signalling and how these phenomena contribute to disease phenotypes.Duties also included supervising and training undergraduate students as well as preparation of scientific manuscripts. -
Postdoctoral FellowHarvard Medical School May 2004 - Apr 2011Boston, Ma, UsSee Instructor in Medicine for details
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Ph.D StudentshipUniversity Of Leicester Oct 2000 - Mar 2004Leicester, GbPerformed research as a graduate student in the field of cell cycle and centrosome regulation. My work focused on how the NIMA kinase Nek2 controls mitotic progression by regulating a novel centrosomal protein called Nlp. We also demonstrated that Nek2B promotes the formation of zygotic centrosomes in Xenopus egg extracts, these in turn are required for early development and highlights a structural function for Nek2B prior to somatic cell division. Duties also included demonstrating experimental techniques to undergraduate students as well as preparation of scientific manuscripts
Joseph Rapley Skills
Molecular Cloning
Cell
Protein Purification
Western Blotting
Protein Expression
Transfection
Cell Culture
Biochemistry
Molecular Biology
Immunoprecipitation
Cell Biology
Fplc
Immunofluorescence
Rnai
Dna Extraction
Rna Isolation
Southern Blot
Cdna Library Screening
Yeast Two Hybrid
Pcr Amplification And Mutagenesis
Sds Page
Antibody Generation/characterisation
Stable Cell Line Generation
Enzyme Kinetics
Lentivirus
Mouse Handling
Elisa
Genetics
Protein Kinases
Adenovirus
Baculovirus
Cart Cell Technology
Humanizing Antibodies
Scfv And Fab Production/purification
Joseph Rapley Education Details
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University Of LeicesterBiochemistry -
University Of SussexMolecular Genetics In Biotechnology
Frequently Asked Questions about Joseph Rapley
What company does Joseph Rapley work for?
Joseph Rapley works for Siemens Healthineers
What is Joseph Rapley's role at the current company?
Joseph Rapley's current role is Manager Technical Operations at Siemens Healthineers.
What is Joseph Rapley's email address?
Joseph Rapley's email address is jo****@****ens.com
What is Joseph Rapley's direct phone number?
Joseph Rapley's direct phone number is +178148*****
What schools did Joseph Rapley attend?
Joseph Rapley attended University Of Leicester, University Of Sussex.
What are some of Joseph Rapley's interests?
Joseph Rapley has interest in Specifically Cycling And Hiking, Outdoor Pursuits.
What skills is Joseph Rapley known for?
Joseph Rapley has skills like Molecular Cloning, Cell, Protein Purification, Western Blotting, Protein Expression, Transfection, Cell Culture, Biochemistry, Molecular Biology, Immunoprecipitation, Cell Biology, Fplc.
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