Joseph Chapo Email & Phone Number

Broomfield CO

Broomfield CO

• DIagnostic Assay Development
• Developed a variety of diagnostic assays from existing critical reagents through all aspects of design control through full commercialization of product
• Responsible for drafting of SOPs, test methods, publication abstracts/posters and commercial product literature
• Responsible for both the project management and development of assay from early stage concept to fully commercialized product
• Experience and continual training in a FDA regulated GMP work environment
• Experience drafting design control documentation with respect to CLSI guidelines

• Perform routine testing and reporting of in-process samples for residual DNA using Real-Time Taqman qPCR
• Design experiments to test and remediate existing and future assays
• Electronic data (Waters Vision Publisher) and sample management documentation (Thermo-SampleManager)
• Writing of Amgen Technical Reports
• Generation, recording and reporting of data in accordance with GLP/GMP guidelines

Develop assays and reagents for testing small molecule inhibitors of targets implicated in cardio-renal disease. Primary responsibility for coordinating and training other team members as well as running gene expression assays (Luminex-Multiplex Assays, Q-RT-PCR) as well as developing new assays to support drug development projects. Luminex Technology..

• Develop assays and reagents for testing small molecule inhibitors of targets implicated in cardio-renal disease
• Primary responsibility was for coordinating and training other team members as well as running gene expression assays (Luminex-Multiplex Assays, QPCR) as well as developing new assays to support drug development projects
• Directly contributed to projects involving HDACi, PKDi, ASK1i, and CAMKi, as well as Endothelin Receptor Pharmacology, Alpha Myosin Heavy Chain and Fibrosis Projects, In-Vivo Pharmacology support, and assay development

Nucleic Acid Isolation and Analysis. DNA and RNA-*Isolation from a broad variety of sources i Familiarity with micro-array set-up and analysis. Cloning experience Design and analysis of several hundred constructs ranging in size from 0.1 to 20 kb. Use of both classical, PCR and oligo-based gene design. Blotting- Northern and Southern Blot analysis.

Conduct research using genetic and molecular biological techniques in the study of apoptosis in Drosophila melanogaster. This work included use of fluorescent microscopy to study X-ray induced GFP expression. Additional work includes real-time PCR and affinity protein purification from radiation responsive genes as well as transfection and maintenance of.

• Implementation and design of the Biotechnology program
• Didactic and laboratory instruction of a broad demographic range of students
• Curriculum encompassed vast scope of biotechnology including: Basic through advanced molecular biology; Forensic Biotech; Bioethics and Industrial Biotechnology
• (Additionally taught 1 semester of Anatomy and Physiology)

Coordinate the daily operations of a molecular biology based pathology lab. Study the transcriptional regulation of type IV collagen. This work used both primary and cultured cells. Developed isolation methods for harvesting both transcriptional active and trans-interactive competent nuclei and proteins. Responsible for training of new personnel, lab.

Phd(Incomplete)

Advanced course work in Cell Biology, Immunology and Molecular Genetics

Ms, Microbiology

B.S, Biology