Joseph Kaszubowski, Phd Email & Phone Number

Boston, Ma, Us

Cambridge, Massachusetts, United States

Dissertation: DNA Polymerase Interactions and Their Role In Translesion Synthesis Substitutions• Worked in the lab of Dr. Michael Trakselis with research focused on DNA replication and repair• Became subject matter expert (SME) on translesion synthesis, DNA polymerase substitution mechanisms, and DNA damage tolerance• Designed protocols for molecular cloning, gene editing, protein expression, and protein purification• Gained experience in molecular biology, plasmid design, PCR, primer design, site-directed mutagenesis, transfection, electroporation, western blotting, and DNA purification• Protein expression involved insect cell culture, bacterial cell culture, and baculovirus expression• Protein purification required AKTA FPLC instrumentation (Unicorn Software), ion exchange chromatography, size exclusion chromatography, and affinity-based methods• Performed ATPase assays, primer extension assays, anisotropy, thin-layer chromatography, radio-labeled DNA extension, flow cytometry, and rapid quench-flow• Data analysis included GraphPad Prism, Microsoft Excel, and JMP• Purified proteins were characterized through enzyme kinetics, protein-protein binding, and protein-DNA binding assays• Received the Graduate Research Productivity Award for the 2021-22 school year

Rensselaer, New York

• Conducted small-scale mammalian (CHO) cell culture experiments to troubleshoot for Clinical and Commercial manufacturing• Monitored cell culture growth, pH, viability (VCD/IVCD), metabolite concentrations, and protein (titer) production through Nova FLEX and HPLC analysis• Worked directly with Clinical Mfg, Commercial Mfg, QC, Downstream Process Sciences, and Cell Banking in a cross-functional environment• Performed raw material screening and qualification to optimize cell culture performance• Performed design of experiments (DoE) and data analysis on fed-batch culture process optimization• Authored summary reports and presented results at cross-functional meetings• Gained exposure to working in a GMP environment incorporating continuous improvement, in-process control (IPC) tests, and Laboratory Information Management Systems (LIMS)• Assisted in root-cause analysis to improve upstream manufacturing process robustness

Rensselaer, Ny

• Supported Upstream Process Sciences by conducting mammalian (CHO) cell culture experiments• Grew and maintained CHO cells from thaw through production and harvest• Small-scale experiments were conducted in shake flasks, WAVE cell bags, and benchtop bioreactors• Experiments were performed in parallel with Clinical and Commercial manufacturing• Learned sterile transfer, media preparation, and raw material screening• Gained experience with both plant-based and chemically-defined media

Undergraduate student researcher in the lab of Dr. Rachel Sterne-Marr. Research focuses included G protein-coupled receptors (GPCRs), GPCR kinases, alpha-2A-adrenergic receptors, receptor desensitization, and antibody characterization.

Loudonville Ny

Doctor Of Philosophy - Phd, Biochemistry

Bachelor Of Science (B.S.), Biochemistry

Activities and Societies: American Red Cross Club of Siena College, Study Abroad at National University of Ireland Maynooth (Spring.