Joseph Kaszubowski, Phd Email & Phone Number

Boston, MA, US

Cambridge, Massachusetts, United States

• Dissertation: DNA Polymerase Interactions and Their Role In Translesion Synthesis Substitutions
• Worked in the lab of Dr. Michael Trakselis with research focused on DNA replication and repair
• Became subject matter expert (SME) on translesion synthesis, DNA polymerase substitution mechanisms, and DNA damage tolerance
• Designed protocols for molecular cloning, gene editing, protein expression, and protein purification
• Gained experience in molecular biology, plasmid design, PCR, primer design, site-directed mutagenesis, transfection, electroporation, western blotting, and DNA purification
• Protein expression involved insect cell culture, bacterial cell culture, and baculovirus expression

Rensselaer, New York

• Conducted small-scale mammalian (CHO) cell culture experiments to troubleshoot for Clinical and Commercial manufacturing
• Monitored cell culture growth, pH, viability (VCD/IVCD), metabolite concentrations, and protein (titer) production through Nova FLEX and HPLC analysis
• Worked directly with Clinical Mfg, Commercial Mfg, QC, Downstream Process Sciences, and Cell Banking in a cross-functional environment
• Performed raw material screening and qualification to optimize cell culture performance
• Performed design of experiments (DoE) and data analysis on fed-batch culture process optimization
• Authored summary reports and presented results at cross-functional meetings

Rensselaer, NY

• Supported Upstream Process Sciences by conducting mammalian (CHO) cell culture experiments
• Grew and maintained CHO cells from thaw through production and harvest
• Small-scale experiments were conducted in shake flasks, WAVE cell bags, and benchtop bioreactors
• Experiments were performed in parallel with Clinical and Commercial manufacturing
• Learned sterile transfer, media preparation, and raw material screening
• Gained experience with both plant-based and chemically-defined media

Undergraduate student researcher in the lab of Dr. Rachel Sterne-Marr. Research focuses included G protein-coupled receptors (GPCRs), GPCR kinases, alpha-2A-adrenergic receptors, receptor desensitization, and antibody characterization.

Loudonville NY

Doctor Of Philosophy - Phd, Biochemistry

Bachelor Of Science (B.S.), Biochemistry

Activities and Societies: American Red Cross Club of Siena College, Study Abroad at National University of Ireland Maynooth (Spring.