- I lead the Anellovector Discovery team that developed the world's first functional gene therapy vector based on the human anellovirus. - Manage a 9 person team- I lead virology projects in basic anellovirus biology to better understand viral protein function and mechanisms of action- I spearhead projects in assay development, process development, and vector production- My engineering efforts have directly resulted in the submission of multiple patent applications and manuscripts

- Designed high throughput screening platform that led to the development of a blood brain barrier crossing AAV capsid (patent pending)- Developed novel nucleic acid detection assays

- Designed, engineered, produced, and screened novel AAV capsid variant libraries in NHP in support of New Capsid Discovery efforts to identify novel AAV capsids for more effective delivery of gene therapy payloads to target tissues.

• Discovered that epigenetic restriction of herpes simplex virus gene expression occurs in a biphasic manner that is ATRX-dependent.•Leveraged CRISPR-Cas9 technology, fluorescence microscopy, ChIP-qPCR, ATAC-seq, ChIP-seq, and RNA-seq to investigate the formation of viral chromatin in human cells.• Engineered an auto-fluorescent herpes simplex virus for live-cell imaging applications.• Experienced in quantitative PCR (qPCR), chromatin immunoprecipitation (ChIP), fluorescence confocal and widefield microscopy with imaging quantitation, cellular engineering, CRISPR-Cas9 gene editing, NGS, RNAi, flow cytometry, molecular virology and molecular biology techniques.http://knipelab.med.harvard.edu/

•Developed qPCR-based assay of trisomy detection/verification•Immortalized human primary fibroblasts with human telomerase using a retroviral vector•Conducted lentivirus delivered shRNA-based tumor cell viability assays under hypoxic conditions•Gained experience with robotic liquid handling, qPCR, E. coli, oncology, and molecular biology techniqueshttp://espinosalab.org/

•Characterized double stranded RNA foci that form in the nuclei of C. elegans with specific mutations in ALS associated genes, such as a deletion of tdp-1, an ortholog of the ALS associated human TDP-43. •Identified critical time points for foci formation and dissipation and a possible link between dsRNA foci formation and the formation of HSF-1 stress granules in C. elegans•Developed technical skills such as immunocytochemistry, fluorescence microscopy, integration, tissue culture, and C. elegans husbandry•Funded by a fellowship award

•Engaged clients to identify how XINET's enterprise level digital asset management platform would meet their business needs•On-site client visits with technical sales staff for live product demonstration and implementation discussions•Vendor/client relationship management•Team lead on RFP projects for Fortune 500 companies•Sourced and cultivated new clients/markets to expand client base

•Client services - order inquiry, cross selling managed properties, order fulfillment•Resolve order related issues- pricing, shipping, quantity, etc.•Establish and maintain client relationships•Exchange balance reconciliation•Heavy client correspondence via email, phone, and fax

• Client services- list and service inquiries•Shipping inquiries• Order and data entry into proprietary software•General office duties as needed by department