Joshua Hines Email & Phone Number

Colorado Springs, Colorado, United States

Protecting and monitoring our water systems is of the utmost importance for the customer as well as the source.

Little Rock, Arkansas Area

o Bio Safety Level 3 (BSL3) laboratory environment▪ Fit tested for N95 mask ▪ Continual work under biological safety cabinets (BSCs)o Intake and processing of mycobacteriology and mycology samples into the laboratory according to SOP for various types of body fluids and tissues▪ Proficient with LIMSo Microscopy and molecular techniques to identify fungi and AFBo Experience with various laboratory instruments and equipment▪ MALDI-TOF MS (Bruker)▪ Gene Xpert (Cepheid)▪ BACTEC MGIT 960 (Bruker)▪ Centrifuges and thermocyclers (Eppendorff, Sorvall, & ThermoFisher)o Maintaining compliance with the College of American Pathologists (CAP) laboratory certification requirements▪ Verification and daily/weekly checks of various regulated metrics● e.g. temperature, humidity, recording lot numbers, receiving reagents, etc.▪ Experience with CAP audit procedureso Extensive use of various staining procedures for mycobacteria and mycology specimens▪ e.g. fluorochrome, Kinyoun, calcofluor white, lactophenol cotton blueo Customer service for submitters to the lab as well as other labs in the lab groupo Ordering laboratory consumableso Writing, editing, and proofreading SOPso Troubleshooting, maintenance, and QC of all laboratory instruments and equipmento Extensive training for HIPAA, cyber-security, and other professional conduct

Little Rock, Arkansas, United States

o Intake, validation, and distribution of COVID specimens from across the state to the testing labo Adhered to QA SOPs and guidelines for human sampleso Troubleshooting issues with specimens, software, and equipment around the laboratoryo Worked closely with other labs and departments to overcome issues with processing sampleso BSL2 laboratory environment under BSCso Underwent safety training for general lab safety and personal contamination

Little Rock, Arkansas, United States

o Worked in the Engineering Division as part of the EPA funded Area Wide Optimization Project (AWOP) team to provide technical assistance to public water systems (PWS) in Arkansas ▪ Worked closely with water operators to ensure compliance with state and federal permit requirements▪ Collected water samples from PWS to verify reported data as well as to troubleshoot problem areas● Handheld analytical meters (e.g. pH probes, turbidimeters, etc.) used on-site as well as sample collection and storage for processing in the ADH labo Data entry and managemento Assisted Engineering staff with inspections and surveys around the stateo Performed routine calibration and maintenance of all lab and field equipmento Duties interrupted due to COVID ▪ Reassigned to Public Health Lab COVID specimen receiving

Little Rock, Arkansas

o Assisted in the investigation of potential cancer clusters by gathering data, generating reports, summarizing available literature, and creating presentation materials o Assisted ACCR personnel with generating reports related to cancer data for public information o Software used included Microsoft 365, SAS, R, & the proprietary cancer database (CancerCORE) o Created, tracked, and edited agreements with vendors supporting ADH o Created and maintained spreadsheets related to ongoing vendor contracts o Maintained professional communication with colleagues, vendors, and the general public

Conway, Ar

o Thesis Title: Optimizing Molecular Methods for Determining Aquatic Hyphomycete Community Membership in a Salinity Gradient: A Microcosm Studyo Laboratory work to culture, extract, and identify fungal communities within a microcosm environmento DNA extraction of fungal samples, PCR, cloning, prep for sequencing▪ Illumina and Sangero Bioinformatics & statistical software experience▪ Mothur, QIIME2, program R

Arkansas Kraft Division - Morrilton, Ar

- Supervise laboratory work involving analysis of wastewater, liquor samples, and other products/byproducts derived from a kraft paper mill- Directly responsible for the onsite wastewater treatment system to ensure NPDES permit compliance - Planning and management of SWPPP, SPC plans (oil & hazardous chemicals), hazardous waste, and onsite landfill - Collection, processing, storage/maintenance, and analysis of data- Presentations to AKD personnel (both mill management as well as hourly employees & contractors) involving training, data analysis, and environmental recommendations- Involved in various projects involving waste stream, wastewater, and solid waste management/mitigation- Problem solving/troubleshooting, professional communication, collaboration with all departments & contractors- Independent project planning, management, and execution for a wide variety of issues

Morrilton, Ar

- Prepared and analyzed wastewater samples for NPDES permit compliance [Conducted analyses for pH, TSS, BOD, COD, and nutrient residuals]- Collected, prepared, and analyzed samples for various byproducts around the mill (e.g. black liquor consistency, sludge consistency, etc.)- Created and maintained the database with all collected data- Completed various projects assigned by department and mill management

Ft Stewart, Ga

- Provided reconnaissance and security for an armored task force.- Maintained all vehicles and weapon systems.- Obtained and maintained all required certifications for job duties.- Map reading skills.- GPS skills.- Worked in all types of weather and terrain.- Honorable Discharge at the rank of E4 (SPC).

Master Of Science - Ms, Biology

Bachelor Of Science - Bs, Environmental Science

Certificate Of Completion, Drum Program