Joubert Banjop Kharlyngdoh, Ph.D. Email & Phone Number

New Orleans, La, Us

New Orleans, Louisiana, Us

Chicago, Illinois, Us

•Designed experiments, projects execution and establishment of animal models for various kidney diseases. •Involved in multiple projects, generating key hypotheses leading to targets validation and completion.•Involved in training of postdocs and supervising of high school students' projects.•Overall research activities helped the lab secured two RO1s funding on viral cytokine storm impact on renal function. Major professional development: Application of extensive in vivo basic research findings to develop therapeutics for different human kidney diseases. Major research achievement: (1) Assessment of sialic acid-based compounds as therapeutics for diabetic nephropathy using ZSF1 rat model.(2) Generation and purification of stably expressed recombinant mutant human Angptl4 protein and assessment for its therapeutic application in glomerular diseases and acute kidney injury. (3) Development of cytokine storm-induced glomerular injury, and assessment of therapeutic antibodies to protect mild, moderate and severe cytokine storms on glomerular and systemic diseases using mice models. (4) Cross species comparative study on the glomerular expression of UDP-GlcNAc 2-Epimerase Kinase (GNE), a key enzyme in sialic acid biosynthesis.Technical skills include Cell culture, Generation of recombinant cell lines, Immunoprecipitation, Fiber cell system, Protein purification (Ni and TALON based system, Sucrose density gradient Ultracentrifugation), Iron and Chloride assays, Electroporation, qRT-PCR, Histological sections and Immunostaining, Confocal laser microscopy, Immunoblotting, 2-D gel electrophoresis, Glomerular Filtration Rate (GFR) measurement (Urine Inulin clearance, and Creatinine assay using Mass Spectrometry), QQQ LC-Mass Spectrometry, Maintenance of transgenic rats and mice, Glomeruli isolation using Dynabeads and Sieving methods, in vivo experiments involving injections (IP, SC, IV, and retro-orbital injections) and microsurgeries with tissues harvest.

New York, Ny, Us

Transcriptional regulation of Glucocorticoid receptor in macrophage biologyMajor professional development:Application of basic research findings to identify Glucocorticoid signaling pathways involved in inflammation and macrophage biology. Significant contribution in research leading to publication as a co-author.Major research achievement: (1) Identification of a novel mechanism to co-immunoprecipitate cofactors protein-protein interaction. (2) Application of lentiviral system for gene disruption and overexpression.(3) Identification of new signaling pathway via co-regulator sites specific phosphorylation that dictates the role of Glucocorticoid Receptor as co-repressor or co-activator. Technical skills include Co-immunoprecipitation, Chromatin immunoprecipitation (CHIP), CHIP-RT-qPCR, Sample preparation for CHIP seq and RNA-seq, Lentiviral gene knockdown, CRISPR/Cas9 Gene knock-in, Mouse bone marrow macrophage isolation, Transgenic mice maintenance and in vivo experiments.

Örebro, Örebro Län, Se

Modulation of androgen receptor function by brominated flame retardants• Development of study plans that include experimental designs and projects execution.• New experimental protocols were developed and established in the laboratory.• Served as a team member in multiple projects by initiating and conducting experiments until completion including data analysis and manuscript writing.• Research findings presented at national and international conferences.• Supervision of High School, Undergraduate and Masters students.• Overall research activities helped the lab to obtain research funding on endocrine disruption.Major professional development:Extensive research work leading to identification of environmental endocrine disruptors that impact male reproductive system and prostate cancer progression. Significant contribution in research leading to publications as a first author and co-author.Major research achievement: (1) Identification of a novel antiandrogenic compounds with potential neuro-endocrine properties. (2) Establishment of recombinant stable cell lines for transcriptional activation assays and GFP-tagged florescence analysis.(3) Identification of the promiscuity of androgen receptor function, harboring point mutations found in prostate cancer, towards compounds exhibiting diastereomeric isoforms. (4) Initiation and development of RT-qPCR array as well as maintenance of Daphnia magna as a model system for studying of endocrine disruption in invertebrate. Techniques: Computational Modeling MOE (Molecular Operating Environment), Cell culture, Site-Directed Mutagenesis, Cloning, Transformation and Transfection, Generation of Recombinant Stable cell lines, siRNA silencing, qRT-PCR, Immunofluorescence, Confocal laser microscopy, Immunoblotting, Development and maintenance of Invertebrate Daphnia magna. Ultra-centrifugation (Isolation of outer membrane vesicles from Gram-positive bacterium Lactobacillus for summer project of a Master student).

Örebro, Örebro Län, Se

Teaching assistant of students at various levels (Masters, Undergraduate and High School) in different courses that cover Cell Biology, Microbial pathogenicity, Model Systems, Molecular Techniques and Drug Development.

In

• Designed experiments, projects execution and establishment of proteomics work in the laboratory.• Development and establishment of new experimental protocols in the laboratory.• Multiple projects involvement by initiating and conducting until completion including data analysis and manuscript writing.• Research findings presented at national and international conferences.• Supervision of Undergraduate and Masters students for summer projects.Major professional development:Application of basic research findings to identify new mechanism of protein secretion in the gram-negative bacterium Vibrio cholerae. Major research achievement: (1) Identification of novel secreted protein profiles between non and virulent strains of Vibrio cholerae. (2) Development and establishment of gene knockout for a highly secreted protease hapA in Vibrio cholerae for better and stable secreted protein profiles. (3) Isolation and identification of outer membrane vesicles as an alternative method of protein secretion mechanism in Vibrio cholerae. Techniques: PCR, Cloning and Subcloning, Gene Knockout using suicide vector approach, Bacterial protein over-expression and purification using His-tagged Column, ELISA, Western Blot, Secreted protein precipitation, Outer membrane vesicles isolation using Ultra-centrifugation, 2-D gel electrophoresis, In-gel and in-solution Tryptic digestion Sample preparation for MALDI-TOF MS/MS analysis, DNA cycle sequencing, Chemical and Biological Sample preparation and analysis using Scanning Electron Microscope, Biological sample preparation and analysis using Atomic Force Microscopy.

Doctor Of Philosophy (Ph.D.), Biology

Master'S Degree, Molecular Biology And Biotechnology

Bachelor'S Degree, Biochemistry