Joao Fonseca Email & Phone Number

Porto, Portugal

- Led a project to enhance internal metabolic modeling capabilities, introducing new algorithms, methodologies, and software solutions.- Partnered in the development of Design of Experiments (DOE) strategies to improve data quality across the organization.- Developed customized dashboards to deliver actionable insights to scientists and stakeholders..

Porto, Portugal

- Collaborated cross-functionally with software engineering, biology, fermentation, and process development teams to deliver integrated solutions.- Designed and developed backend microservices and frontend components for an internal web application used in metabolic modeling.- Contributed to the full-stack development of internal applications supporting.

San Francisco

• Prof. El-Samad Lab
• Designed and conducted experiments in a team setting to create a mammalian cloning toolkit for the rapid and modular design and construction of synthetic gene circuits.
• Designed and conducted experiments in a team setting to create a CAS9-based synthetic circuit capable of controlling the mean and noise of expression of endogenous genes in mammalian cells.

San Francisco

• Prof. El-Samad Lab
• Used a combination of molecular, biochemical, optogenetic, tissue culture, genome editing, next-generation sequencing, microscopy, flow cytometry, and computational techniques to address a wide range of research.
• Independently designed and conducted experiments to identify fundamental rules of cAMP signaling in kidney epithelial cells.
• Designed and conducted experiments in a team setting to create synthetic epithelial tissues that are responsive to light and able to generate highly localized intracellular signals.
• Designed and conducted experiments in a collaboration setting to generate yeast mutants and measure the effect of calcium absence in their growth.
• Co-wrote an NSF-funded grant to drive a new effort in multicellular models research in the lab.

Vienna

• Dr. Ringrose Lab
• Designed and conducted experiments in a team setting to identify modifiers of the kinetic properties of Polycomb Group Proteins in Drosophila stem cells.

Vienna

• Dr. Ringrose Lab
• Used molecular, biochemical, flow cytometry, live-cell microscopy, computational modeling, and fly genetics to address the kinetic properties of PcG proteins in Drosophila.
• Independently designed and conducted experiments to identify the kinetic properties of Polycomb proteins in Drosophila stem cells.
• Mentored master students.
• Interpreted results, summarized data, prepared figures, and wrote a dissertation and successful manuscripts for publication.
• Presented results in lab meetings, national, and international conferences.

Vienna

• Dr. Ringrose Lab
• Used bisulfite sequencing and immunoprecipitation techniques to measure DNA methylation in mouse stem cells
• Performed research in a team to detect changes in DNA methylation of mouse stem cells upon differentiation.

Oeiras

• SOS response in S. aureus, performed in Dr. Mariana Pinho's lab
• Used molecular cloning and live-cell microscopy to measure transcriptional responses of S. aureus to antibiotics.

Lisbon

• Roles of actin in gene regulation, performed in Joao Ferreira's lab
• Used small molecule inhibitors, immunoblotting, and immunofluorescence techniques to measure gene activity in mammalian cell culture in response to disruption of actin polymerization.

London, United Kingdom

• Dr. Michael Antoniou Lab
• Performed research in team generating integration-defective lentiviral vectors for muscle gene therapy.
• Used molecular cloning and southern blotting to generate and quantify the effectiveness of new integration-defective HIV-1 based vectors for muscle gene therapy.

Dr. Rer. Nat. (Doctor Of Natural Sciences), Molecular Biology

Bachelor'S Degree, Cell/Cellular And Molecular Biology