Jan Kostecki, Ph.D. Email & Phone Number

Austin, Texas, Us

Thousand Oaks, Ca, Us

Specialist Information Systems – Business Systems Analyst (May 2015 - December 2019)Product Owner – Molecule Discovery – Biologics- Responsible for supporting, developing and prioritizing Biologics business needs for 13 core Research IS applications spanning computational protein design, LIMS, Sanger and NGS sequencing, Biologics registration and search.- Mentoring, managing and developing staff members in supporting core business critical applications.Business Analyst – High Throughput Biologics (HTB) Project- Developed HTB as the single source for all Biologics data created at AMGEN, from molecule design to protein production. - Leveraged existing commercial (e.g. Geneious) and custom built (e.g. Ab Initio, BioReg) applications to address technical scientific functionalities while integrating with the HTB database to use a standardized set of identifiers. - Standardized requesting and tracking of external vendor services (e.g. GENEWIZ), which eliminated user entry error and provides financial transparency by User, Cost Center and Project. - Leading LIMS application upgrade and migration to AWS including containerization of 70 custom developed applications. Business Analyst – Application Owner – Electronic Lab Notebook- Responsible for supporting and maintaining IDBS E-WorkBook (GxP and non-GxP) and Perkin Elmer E-Notebook.- Leading team of 3 AMGEN FTE and 4 CTS EW resources for global run support of more than 1000 users.Change Management and Research IS Application Training Lead- Identified Biologics Super-Users and utilized train-the-trainer methods to onboard over 550 users within Therapeutic Discovery, Process Development and external service provider (Syngene).Business Analyst Community Lead- Instructor for AMGENs BA Boot Camp; four-day immersive training course covering best practices of business analysis.- Created curriculum and lead training sessions for 70+ business analysts across all of AMGEN’s IS functions.

Pasadena, Ca, Us

Staff Scientist, Laboratory of Professor Stephen Mayo, Biology and Biological EngineeringResearch focused on experimentally validating proteins designed through Triad - a state-of-the-art computational protein design (CPD) software package from Protabit, LLC. Antibody Engineering ProjectsBispecific hetero-dimer CH3 engineering assayed by yeast-2-hybrid selections to identify novel interface variants. Broadly neutralizing antibody engineering against pandemic influenza - a collaborative project with Professor Pamela Bjorkman sponsored by DARPA; utilized yeast display to identify antibodies with increased affinity and breath to various HA subtypes from antibody libraries designed by CPDBiofuel and Natural gas-to-Liquid (GTL) fuel ProjectsIdentified cellulosic enzymes engineered by CPD for increased thermostability in a high throughput screening assay. Thermostable variants were produced in Pichia pastoris, purified to homogeneity, characterized biophysically and for their ability to maintain activity on real-world substrates.Accessory enzymes (e.g. polysaccharide mono-oxygenases) for cellulose degradation were also engineered for thermostability and shown to have a synergistic effect on the release of glucose when combined with a mix of thermostabilized cellulase enzymes. Engineered a stable, soluble and active methane mono-oxygenase under a collaborative project with Professor Amy Rosenzweig's Laboratory at Northwestern University. Responsibilities in addition to the research listed above included hiring/training/mentoring laboratory technicians, budgeting and accounting for laboratory expenses, grant writing, procurement of capital equipment, establishing a LIMS between the collaborative groups, generating timelines with deliverables, identifying solutions to problems encountered during experiments and presenting outcomes from research to collaborators/funding agencies/corporate partners.

Pasadena, Ca, Us

Scientific Consultant to Bary Olafson, CEO, Protabit, LLC.Served as a non-paid scientific consultant and experimentalist while evaluating Triad, a state-of-the art computational protein design software package. Contributed to SBIR and STTR grant applications that were funded for research focused in the areas of cellulosic ethanol and natural gas-to-liquid fuel production. Coordinated multiple parallel projects funded by Protabit at Caltech; maintained project budgets, established timelines and generated experimental deliverables. Presented experimental results on a weekly basis and provided constant feedback on the performance of projects. Advised the company on a wet-lab build out at the Pasadena Biosciences Collaborative.Provided guidance and training to Protabit scientists in state of the art molecular biology techniques along with transferring SOPs for CPD library generation.

Monterey, Ca, Us

Lead scientist in the research, development and commercialization of ProCode: a bacterial surface display antibody platform for diagnostic and therapeutic applications. Involved in all aspects of platform design: antibody library creation and diversification, modular vector design, antigen production and screening, assay development, antibody affinity characterization using Biacore, affinity maturation techniques and in vivo cell based testing. Experienced in project management, hiring/training/mentoring scientists, coordinating and communicating with clients, establishing corporate partnerships and marketing the capabilities of the platform at scientific conferences.

Riverside, Ca, Us

Undergraduate Research Assistant to Professor John M. Hearty, College of Natural and Agricultural Sciences, Entomology. Isolated and sequenced genomic DNA (28S-D2, -D3, 18S-E23 rDNA) from parasitic hymenoptera for phylogenetic classification.Experienced in fieldwork for sample collection, preservation and characterization.

Berkeley, Ca, Us

Undergraduate Research Assistant in Professor George Roderick's Laboratory - Environmental Science, Policy and Management. Mentored by Sean Schoville in phylogenetic analysis and DNA sequencing utilizing ABI Prism 377 DNA Sequencer.

Doctor Of Philosophy (Ph.D.), Biomedical Engineering

Bachelor'S Degree, Bioengineering