Julia Baaske Email & Phone Number

Freiburg, Bw, De

Idar-Oberstein

Preparation and presentation of strategic recommendations to clients. This included qualitative and quantitative asset and target analysis, with a focus on scientific and (pre-)clinical data, and their integration into the business and market landscape.

Driving an agile cross functional project to develop genetically modified protein variants to better understand the structure function relationship of a potential drug candidate. Designing and developing experimental and analytical workflows.Driving process development for protein production (high-density perfusion reactors) and purification (affinity based chromatography AKTA systems).Supporting bioassay development in a GxP environment.Third party management by establishing confidentiality agreements, as well as point of contact for scientific progress.Contributed to assessment of critical quality attributes (CQA) of drug candidate. Test and evaluate application of new software (BioProdict.com) to support CQA assessment.Supervision and training of Master student.Initiation of PostDoc network within department and organization of lecture series (with over 140 participants).Active engagement in employee organization groups and curiosity ambassador for department.

Applying synthetic biology to protein engineering for intra-and extra-cellular control of cellular processes and in vitro diagnostics- Design and cloning of engineered proteins into vectors for expression in mammalian or bacterial cell culture- Routinely performed small-scale protein production in E.coli- Optimization of protein production in different E.coli strains of newly engineered proteins- Routinely performed biochemistry analysis methods such as Western blots or coomassie gels.- Working with different mammalian cell lines and various cell culture techniques including different transfection techniques, production of virus vectors and cell sorting (FACS)- Routinely performed live-cell flow cytometry analysis, live-cell microscopy cell imaging or analysis with confocal microscopeManaged:- supervision of Master and Bachelor students including planning, supervising and discussing projects- organization of several practical courses- planning and giving lectures for students- responsible for the introduction and maintenance of several machines in labPresented:- Presentation of scientific results for PhD defense (resulted in very good/ magna cum laude)- Scientific results as a poster at GASB II conference in Berlin 2018- Routinely presentations of results obtained throughout projects

Validation of the CRISPR/ Cas9 D10A method for ESA and CD44 in cancer stem cells- Working with different mammalian cell lines, including primary cells from breast cancer xenografts- Design and cloning of CRISPR/ Cas9 D10A vectors for expression in mammalian cells- Routinely performed Western Blot and flow cytometry analysis- Hands- on experience with cell sorting using FACS- RNA expression analysis using qPCR- Presentation of results for the company

Milton Park

During my internship at Oxitec I worked on two projects: - Development of injection protocol for Lucilia sericata - Gene disruption in Aedes aegypti using TALE nucleases- Developing insecting rearing protocols

Strasbourg

The project I was working on was aimed to compare protein enrichment involved in cytoskeleton organization at the leading edge of migrating fibroblasts.- confocal and TIRF microscopy- mammalian cell culture

Diploma, Coaching And Psychology

Doctor Of Philosophy - Phd, Biology, Magna Cum Laude

Master Of Science (M.Sc.), Biotechnology, With Honors, Graduated Ranked First Out Of 41

Activities and Societies: Active member of the students association "Amicale" which takes care of organizing events like hiking.

Master Of Science (M.Sc.), Structural Biology And Bioinformatics, Graduated With Honors

as secondary studies

Bachelor Of Applied Science (B.Sc.), Biology, 1,7

with focus on synthetic biology