Julia Torvi Email & Phone Number

Seattle, Washington, United States

Microscope sales specialist helping folks determine, obtain, and build microscope systems to best address their research needs. Covering the PNW including Washington, Idaho, and Alaska.

Seattle, Washington, United States

Projects: Initiated the development of 10 new CRISPR/Cas9 edited cell lines • Delivered CRISPR/Cas9 machinery via both transfection and AAV6 transduction, optimizing conditions for high HDR• Performed stringent quality control to ensure correct editing (ex. ddPCR, PCR, karyotyping, growth curve, flow cytometry)Team management: Co-managed a team of 6 Research Associates• Trained members of the team in technical skills such as gene editing, transfection, hiPSC cell culture, cloning, and QC assays• Project Managed (Jira, Smartsheet, Notion) ~30 cell lines in production at once, setting team goals, evaluating bandwidth, and working with other team leads to determine potential next cell lines• Developed and implemented standardized organizational systems including electronic lab notebooks (OneNote) and data management (Sharepoint)

Advisors: Georjana Barnes, Ph.D. & David Drubin Ph.D.Project 1: Developed a microscopy based reconstitution assay to detect and quantify novel mitotic protein dynamics• Uncovered novel roles for a kinesin-8 and a microtubule polymerase in chromosome congression• Applied TIRF microscopy to quantify protein dynamics and utilized mass spectrometry to analyze protein abundance,uncovering novel cell-cycle regulators of microtubule dynamics• Built a micropatterning system to control microtubule geometry on a coverslip to better reconstitute spindle-like architecture Project 2: Initiated an external collaboration resulting in the discovery of novel functions of a microtubule polymerase• Uncovered novel essential domains of a microtubule polymerase using targeted mutational analysis and subsequent directvisualization of protein activity on the microtubule using TIRF microscopy• Awarded CRCC Graduate Research Fellow grant given to 5% of applicants • Received NSF GRFP Honorable Mention given to 15% of applicants

Advisor: Sue Biggins, Ph.D.Project 1: Independently led a research project resulting in the development of a protocol to purify mammalian kinetochores • Stably integrated epitope tags into the genome of mammalian cells grown in-suspension for protein complex purification Lab management: Supervised the lab’s databases, supplies, and logistics to keep the lab running smoothly• Trained members of the lab on microscope, tissue culture, and other equipment usage

Advisor: Jason Stumpff, Ph. D.• Engineered a FRET-based tension sensor to quantifiably measure kinetochore forces during cell division • Developed a NIS Elements and Fiji based pipeline to quantify forces at the kinetochore in live cells• Awarded Robert Woodworth Award for Research Support given to 15% of applicants • Awarded NSF Robert Noyce Summer Scholarship given to 5% of applicants

Doctor Of Philosophy - Phd, Biophysics

Bs, Biochemistry

High School Diploma