Established and led yeast display lab for Immune checkpoint antibody selectionEstablished high efficiency yeast transformation for immune library with high diversityEstablished and led Protein purification unit:- Generation of MHC class I for TCR selection- Established UV-mediated peptide exchange for on-demand generation of MHC class I with diverse epitope- Established assay to assess the peptide specificity of MHC class I

Constructed a human proteome microarray & established protocols to significantly increase the throughput and the yield- Completed high-throughput expression clone generation (~34,000 clones)- Completed high-throughput protein purification (~17,000 human proteins) through high-throughput yeast transformation and protein over-expression system (>90% detectable by Western blot)- Increased protein production by > 4 times higher over the yearsPerformed antibody and antigen selection using proteome microarray and validation by overexpression and RNAi approach to determine and assess specificity of ~50 uncharacterized monoclonal antibodies generated by a shotgun approach, leading to commercialization (CDI Laboratories, Puerto Rico)- Collaborated on Biomarker discovery (serum profiling for auto-antigens in cancers and autoimmune diseases)- Collaborated on functional dissection of ubiquitin E3 ligase enzyme assays with Ube3A and substrate catalogingCollaborated on HIV-Human protein interactomeCollaborated on proteome-wide SUMO interactome and SUMOylome Performed protein-lipid interaction and protein-drug interaction (artemisinin) screeningCollaborated on high-resolution proteomics investigation of Parkinson’s disease protein interactome (PDome) using mass spectrometry and protein microarraysCollaborated on protein nitrosylation and acetylation of human proteome

Scientific/technical Manager of protein microarray core and research collaborations - Established and maintained quality control of protein microarray production- Trained and supervised a staff for protein array production as a technical manager- Investigated protein interactions with p53/p63/p73 to define chemoresistance mechanism in cancer- Collaborated on proteome-wide poly-ADP-ribosylation (PAR) modification by PARP-1 enzyme and PAR binding and its interaction with ubiquitin pathways