Responsible for new assay development--personalized precision medicineservice and new product development using PDX and miniPDX model.

Immunoassay using AlphaLISA technology for commercial assay kit development targeting cancer, immuno-oncology, inflammation, asthma, metabolism and etc. Set up enzymatic assay for protease activatable optical imaging probe development including MMPs and CathespinB for cancer and caspases for apoptosis, carbonic anhydrase IX for hypoxia, cathepsin K for osteoporosis, renin for hypertension, neutrophil elastase for acute lung injury. Assay development for inhibition of caspases with small compounds. Expression and purification of recombinant proteins in mammalian cells and E. coli. Identification of a novel inhibitor of protein phosphatase1 (inhibitor-3) using the yeast two-hybrid system. Cell Based Assay: Successfully develop celluar assay with AlphaLISA technology especially for epigenetics markers as H3K4me3, H3k9me3, H2AK119ubiquitin. Set up cellular models for screening caspase inhibitors and Annexin V for apoptosis detection. Set up cellular uptake of Near Infrared protease activatable probes with fluorescent microscope. Ex-vivo target validation: Set up in-situ enzymatic tissue staining to access protease activity in diseased animals to validate the target and mechanism of action. In vivo animal imaging, Gene Therapy and Pharmacological Analysis: Non-invasive animal imaging studies with protease activatable and targeted probes to monitor disease progress and xenograft tumor in mice with VisEn Fluorescence Molecular Tomography (FMT). Construction of retroviral vectors (RV) expressing human factor IX; Development of an immunocytochemistry method to titer RV, neonatal mice treatment with retroviral vector; Detecting and monitoring the expression level of human factor IX and anti-human factor IX antibodies formation in mouse and dog using self-developed ELISA; Evaluation of the efficacy of caspase-3 inhibitor on sepsis animal model. Analysis of active PARP-1 in pancreas in diabetic rats induced by streptozotocin.

propose a research project, coordinate a program, design experiments, perform experiment procedures, collect and analyze data and hunt problems.

Design in vitro and in vivo experiments for biochemical, cell-based assay and small animal optical imaging for biomarkers involved in diseases like cancer, inflammation, hypertension, osteoporosis, hypoxia and apoptosis.