Junwei Yu Email & Phone Number

San Diego, CA, US

San Diego, California, United States

• Cultivate and maintain various cell types, including cell lines, iPSC and primary cells.
• Isolate, activate and expand primary T cells. Construct Lentivirus plasmids, produce lentivirus particles and transduce primary T cells, eventually generating potent CAR-T cells.
• Extensive experience with diverse delivery technologies, including transfection, nucleofection, and viral transduction.
• Proficiently design sgRNAs and HDR donors for CRISPR-Cas9 gene editing, leading to the successful generation of numerous knockout (KO) and knock-in (KI) cell lines, including iPSC with high-throughput screening.
• Design primers & probes and conduct ddPCR, RT-qPCR to determine DNA and RNA level changes and execute Western blot and flow cytometry analyses to investigate and quantify protein level changes.
• Perform advanced cell assays, such as Cell-Titer-Glo Cell Viability Assay, GSH/GSSG-Glo™ Assay.

Waltham, Massachusetts, United States

• Constructed a wide range of complex plasmids using diverse methods, including traditional restriction enzymes, HiFi DNA assembly, and Gateway cloning.
• Generated numerous knock-in (KI) and conditional/inducible knock-out (KO) fruit fly lines using CRISPR-Cas9 based gene editing method.
• Conducted research on the influence of the circadian rhythm system on sleep and learning memory performance with a comprehensive array of techniques, such as immunofluorescence (IF), confocal imaging, live calcium.
• Explored the functional roles of proteins of IA-2 and its interactors in the regulation of DCV's (dense core vesicle) generation, budding, sorting, maturation, and transportation by utilizing Co-IP.
• Served as the senior laboratory technical advisor, offering invaluable guidance and mentorship to undergraduate students, graduate students, and research assistants.