Development of AAV-based gene therapies for cardiovascular diseases- supervision of research technicians and scientists for lead product development (AAV vector manufacturing (upstream-/downstream processing), quality control, biodistribution analysis, seroprevalence etc.)- lab organization, implementation of lab automation , ELN protocols and scientific reporting- coordination of inhouse R&D activities and contract research organisations- project leader for biological safety and occupational safety officer

Development of heart-specific AAV for gene therapy of cardiovascular diseases- Generation, validation and screening of highly diverse viral libraries- Production, purification and characterization of AAV vectors- nucleic acid extraction from tissues for NGS and data analysis with Python- Assay development (lab automation, digital PCR and ELISA)- supervision of research technicians and scientists- scientific interaction with CROs- project leader for biological safety- occupational safety officer

Project: "Proteolytic regulation of microtubuli-dependent processes in human cells"- Leading a team of master students and one research technician - Protein expression, purification and characterisation from bacteria, insect and human cells via FPLC (IEX, SEC, Affinity), such as complex neddylated Cullin-RING ligases- in vitro application of posttranslational modifications on recombinant proteins (Ubiquitin, Nedd8)- cell line generation via CRISPR/Cas9-based genome editing, retroviral gene transfer (biosafety level S2) and FACS- antibody affinity purification and characterisation- cell proliferation and toxicity assays in high throughput- imaging (Super-resolution (STED), confocal, spinning disc, wide-field)- supervision of antibody practical for master students- supervision of master students during lab rotations

Project: "Regulation of Kar9 by SUMO-targeted ubiquitin ligases in Saccharomyces cerevisiae".- live-cell fluorescence microscopy- visualization of protein-protein interaction in living cells- protein expression, purification and characterization from S. cerevisiae and E. coli- analysis of posttranslational protein modifications (in vitro and in vivo)- molecular cloning using Gibson assembly and standard restriction enzyme based strategies- supervision of bachelor students

Project: "Regulation of Kar9 by SUMO-targeted ubiquitin ligases in Saccharomyces cerevisiae".- live-cell fluorescence microscopy- visualization of protein-protein interaction in living cells- protein expression, purification and characterization from S. cerevisiae and E. coli- analysis of posttranslational protein modifications (in vitro and in vivo)- molecular cloning using Gibson assembly and standard restriction enzyme based strategies- supervision of bachelor students