Kai-Ti Chang, Ph.D

Kai-Ti Chang, Ph.D Email and Phone Number

Scientist at Regeneron @ Regeneron
Kai-Ti Chang, Ph.D's Location
White Plains, New York, United States, United States
Kai-Ti Chang, Ph.D's Contact Details

Kai-Ti Chang, Ph.D work email

Kai-Ti Chang, Ph.D personal email

n/a

Kai-Ti Chang, Ph.D phone numbers

About Kai-Ti Chang, Ph.D

I am a specialist in mitochondrial biology, protein biochemistry and cell biology. My research has focused on the understanding of molecular mechanism underlying fundamental processes involved in viral maturation or cell death using biophysical and biochemical techniques.• Overexpress recombinant protein in E. coli, insect cells or mammalian cells.• Utilize AKTA system with various columns to purify target proteins.• Mastered a variety of molecular, protein chemistry, and cellular methods, including mammalian cellculture, transfection, flow cytometry, immunocytochemistry, and western blotting.

Kai-Ti Chang, Ph.D's Current Company Details
Regeneron

Regeneron

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Scientist at Regeneron
Kai-Ti Chang, Ph.D Work Experience Details
  • Regeneron
    Scientist
    Regeneron Jun 2022 - Present
    Tarrytown, New York, Us
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  • Integral Molecular
    Research Scientist
    Integral Molecular Jun 2021 - Jun 2022
    Philadelphia, Pennsylvania, Us
    Develop polishing steps for bispecific antibodies and characterize their biophysical properties
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  • Euprotein Inc.
    Senior Scientist
    Euprotein Inc. Jun 2020 - Jun 2021
    North Brunswick, Nj, Us
    - Design and execute experimental plans, write SOPs and reports as well as train junior Scientists. - Perform mammalian cell (HEK293) culture and transfection.- Conduct and optimize expression and purification of target proteins via different chromatography using AKTA system. (Resulted in a 50 % increase of protein yield. Up to 20 L)
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  • Rowan University
    Postdoctoral Research Fellow
    Rowan University Feb 2017 - Jun 2020
    Glassboro, Nj, Us
    - Transiently transfected (electroporation or liposome) mammalian and human tumor cells to understand the molecular mechanism with oxidative stresses through a variety of cellular and molecular assays, including CRISPR, immunocytochemistry, multicolor flow cytometry, MTT assay, wester blot, immunoprecipitation, pull down assays and Seahorse assays.- Studied protein-protein interactions in vitro (protein expression and purification, site-directed mutagenesis, size exclusion chromatography, - Experience in gene editing (CRISPR), stable cell line establishment (human primary/tumor cells) and lentivirus transduction, qPCR and RNA isolation.
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  • University Of Maryland
    Research Assistant
    University Of Maryland Aug 2009 - Jan 2017
    College Park, Md, Us
    - Studied the mechanism of a lipid channel in apoptosis with electrophysiology, isolated mitochondria, mouse handling and protein purification. - Investigated protein-lipid interactions (molecular docking, site-directed mutagenesis, and fluorescent quenching). - Expressed and isolated an human enzyme from insect cells (SF9) and determined its steady-state kinetic parameters.
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  • University Of Maryland
    Graduate Teaching Assistant
    University Of Maryland Sep 2008 - Dec 2015
    College Park, Md, Us
    Mentored 600+ students in Cell Biology/ General Chemistry Courses as Teaching Assistant
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  • Academia Sinica, Taiwan
    Research Assistant
    Academia Sinica, Taiwan Apr 2007 - Jul 2008
    Taipei City, Taipei City, Tw
    - Utilized isotope labeled amino acids to label proteins that expressed in mammalian cancer cells. Utilized mass-spectrometry-based proteomics to identify and quantify the proteins.
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Kai-Ti Chang, Ph.D Skills

Research Microsoft Office Biochemistry Cell Culture Pcr Protein Chemistry Matlab Powerpoint Molecular Biology Microsoft Excel Protein Purification Protein Expression Data Analysis Science Statistics Western Blotting Molecular Cloning Microsoft Word Visual Basic Proteomics Polymerase Chain Reaction High Performance Liquid Chromatography Mass Spectrometry Proteo Assay Development X Ray Crystallography Electrophysiology Patch Clamp Sds Page Fplc Protein Protein Interactions Microsoft Powerpoint

Kai-Ti Chang, Ph.D Education Details

  • University Of Maryland
    University Of Maryland
    Biochemistry
  • National Taiwan University
    National Taiwan University
    Biochemical Sciences
  • National Taiwan University
    National Taiwan University
    Agricultural Chemistry

Frequently Asked Questions about Kai-Ti Chang, Ph.D

What company does Kai-Ti Chang, Ph.D work for?

Kai-Ti Chang, Ph.D works for Regeneron

What is Kai-Ti Chang, Ph.D's role at the current company?

Kai-Ti Chang, Ph.D's current role is Scientist at Regeneron.

What is Kai-Ti Chang, Ph.D's email address?

Kai-Ti Chang, Ph.D's email address is kc****@****lar.com

What is Kai-Ti Chang, Ph.D's direct phone number?

Kai-Ti Chang, Ph.D's direct phone number is +130191*****

What schools did Kai-Ti Chang, Ph.D attend?

Kai-Ti Chang, Ph.D attended University Of Maryland, National Taiwan University, National Taiwan University.

What skills is Kai-Ti Chang, Ph.D known for?

Kai-Ti Chang, Ph.D has skills like Research, Microsoft Office, Biochemistry, Cell Culture, Pcr, Protein Chemistry, Matlab, Powerpoint, Molecular Biology, Microsoft Excel, Protein Purification, Protein Expression.

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