Joshua Kane Email and Phone Number
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Mission-driven drug discovery scientist with 7+ years industry experience strongly committed to addressing unmet medical needs, with expertise in project team leadership, induced proximity platforms (including targeted protein degradation), oncology and infectious diseases indications, mammalian cell culture, molecular biology, and data analysis.• Highly experienced in mammalian cell culture (primary and cell lines), cell and molecular biology, virology, automated microscopy-based assays, mode-of-action validation studies, and data analysis.• Expertise in study design, execution, analysis, and presentation, with adherence to project timelines.• Biology team leader on multiple drug discovery programs in highly cross-functional team environments• Supervising research team• Excellence in communication, collaboration, and writing, with strong emphasis on team-oriented, milestone-driven activities• Effective in hybrid work environments (proficiency with Microsoft Teams, Zoom, GoToMeeting)** Technical expertise **__Cell culture__• Mammalian cell culture (primary cells and cell lines) • Lentiviral transduction • Virology• RNAi / CRISPR interference (CRISPRi) • Targeted protein degradation• Drug treatment / dose-response assays / rescue studies / viability studies• MOA determination / validation studies• Transfection (transient and stable)• Cell line generation• Clonal line development / isolation / expansion • Plate-based immunofluorescent assays (ImageXpress) • Multicolor flow cytometry • in vitro click chemistry__Molecular biology__• PCR and RT-qPCR • Primer design • Cloning • Site-directed mutagenesis • Plasmid construction / design / synthesis• cDNA generation • Western blotting / immunoblotting• Automated immunoblotting (ProteinSimple Jess)• Lysate fractionation • Virus generation and purification__Biochemistry and biophysics__• Protein affinity purification (native, denaturing, tandem affinity purification) • Protein quantification (BCA)• Protein dialysis / buffer exchange • in vitro labeling (biotin, dyes, click chemistry reagents) • SPR (Biacore T100/200, 8K) • BLI (Octet) • Protein-compound and protein-protein binding assays__Data analysis__• High proficiency with Excel, Python, Prism Graphpad, and R (incl. Shiny app development) • High proficiency with platform-specific data analysis software (XLFit, Cellprofiler, Biacore evaluation software, ForteBio data analysis). • Experienced in analyzing public and proprietary datasets, including proteomics and gene knockdown/knockout datasets.
Nurix Therapeutics
View- Website:
- nurixtx.com
- Employees:
- 368
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Nurix TherapeuticsSan Francisco, Ca, Us -
ScientistNurix Therapeutics Aug 2020 - PresentSan Francisco, Ca, Us -
Postdoctoral FellowNovartis Institutes For Biomedical Research (Nibr) Dec 2016 - Jul 2020Basel, Baselstadt, Ch• Characterized anti-viral compounds targeting BK and JC polyomaviruses, including determining efficacy (EC50, CC50) and developing exploratory assays to detail mechanism of action. • Composed manuscript (first-author) describing anti-viral peptide targeted polyomavirus capsid (in revision; pre-print on bioRxiv).• Validating and profiling early-stage compounds using SPR (Biacore T100/200 and 8K) and BLI (ForteBio Octet) biophysical platforms, performing protein-compound and protein-protein binding assays, optimizing relevant assays.• Contributing to active projects as team member, presented efforts in local project-team, site-wide, and inter-site meetings.• Engaged in leadership role as site postdoc representative. -
Postdoctoral ResearcherUcsf Sep 2015 - Nov 2016San Francisco, California, UsKrogan Lab, UCSF Mission BayProject: Identify Novel HIV-Host Interactions• Identified novel HIV Vif E3 ligase targets using global ubiquitylation profiling (Ubi-Scan) and performing subsequent data analysis using in-lab built tools.• Collaborated on CRISPRi-based HIV genetic screening and interaction profiling platform in human T-cell lines (CEMT4, SupT1).• Optimized large-scale harvesting of HIV-1 virus and enrichment of infected cells for genetic screen -
Phd StudentUcsf Sep 2009 - Sep 2015San Francisco, California, UsResearch Advisor: Dr. Nevan J. Krogan• Investigated evolution of host-viral interactions, leveraging affinity purification-mass spectrometry technology, using HIV-1 Vif E3 ligase as model.• Validated findings with follow-up investigation using multiple mammalian (human and non-human) cell lines, small-molecule inhibitors, and RNAi.• Discovered lineage-specific, consequential viral-host interactions in Lentivirus genus and published results as first-author in Cell Reports (video abstract: youtu.be/pRMoXLJKPNc).• Key leadership role in guiding international collaboration at UCSF, U. of Minnesota, and U. of Iceland. -
Undergraduate Student ResearcherUniversity Of California, Berkeley 2006 - 2009Berkeley, Ca, UsResearch Advisor: Dr. Michael Freeling• Developed with graduate student mentor web-based comparative genomics platform CoGe (http://genomevolution.org/coge/); accomplishments include designing front-end web interface, coding Perl-based wrappers for established genomics tools, and setting up documentation wiki.• Led investigation, under own initiative, into the evolution of a tandem gene array in A. thaliana and A. lyrata using CoGe platform and other phylogenetics tools.• Published undergraduate research as first-author in Journal of Molecular Evolution.
Joshua Kane Skills
Joshua Kane Education Details
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University Of California, San FranciscoBiochemistry And Molecular Biology -
University Of California, BerkeleyBiochemistry And Molecular Biology
Frequently Asked Questions about Joshua Kane
What company does Joshua Kane work for?
Joshua Kane works for Nurix Therapeutics
What is Joshua Kane's role at the current company?
Joshua Kane's current role is Senior Scientist at Nurix Therapeutics.
What is Joshua Kane's email address?
Joshua Kane's email address is jk****@****inc.com
What schools did Joshua Kane attend?
Joshua Kane attended University Of California, San Francisco, University Of California, Berkeley.
What are some of Joshua Kane's interests?
Joshua Kane has interest in Education.
What skills is Joshua Kane known for?
Joshua Kane has skills like Bioinformatics, Proteomics, Molecular Biology, Western Blotting, Protein Expression, Python, Mammalian Cell Culture, Affinity Purification, Rnai, Comparative Genomics, Evolutionary Biology, Crispr Interference.
Who are Joshua Kane's colleagues?
Joshua Kane's colleagues are Tim Kane, Aaron Moranville, Caroline Davis, Kevin Miller, Mary Daniel, Janna-Elea Viray, Adrienne L..
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