Brian Kang Email & Phone Number

• Design and perform studies to identify lead compound and progress through candidate selection: these activities include performing in vitro studies such as minimum inhibitory concentration (MIC) assay/MIC-lowering assay, microsomal stability assay, and biofilm assay• Perform activities to support biological studies for NIH contracts, internal research projects, and CRO studies• Collaborate with CRO to effectively carry out ADME, PK, and toxicology assessments in rodents• Carry out bioanalytical method development, validation, and sample analysis using LC-MS/MS• Perform activities to support the chemical synthesis of compounds for Agile Sciences' medicinal chemistry program and other chemistry needs and manage medicinal chemistry databases• Develop, troubleshoot, and prepare drug formulations• Practice quality control standards• Update management of scientific progress in verbal and/or written form• Coordinate junior research assistants and technicians and ensure all tasks are completed on schedule• Maintain a safe laboratory working environment

Morrisville, Nc

• Project leader for host resistance and immunotoxicology studies that implement standard operating procedures (SOP) and good laboratory practices (GLP)• Coordinate research technicians and interns and ensure study tasks such as reagent preparation, animal doing, and organ collection are carried out according to SOP and GLP guidelines• Budget and order animals and reagents necessary for studies and prepare test articles according to study protocol• Prepare data entry forms and establish templates to facilitate data entry and analysis• Verify data entry, perform calculations and statistical analyses, and present final data package to senior management in oral or written formats• Identify potential quality control (QC) or study protocol failures and notify quality assurance officer and study director

Morrisville, Nc

• Performed preclinical host resistance and immunotoxicology assessments that implemented standard operating procedures (SOP) and good laboratory practices (GLP)• Participated in host resistance and infectious disease models via animal receipt and randomization, animal weight documentation, infection, dose (subcutaneous/intraperitoneal/oral gavage/intravenous), euthanization via CO2, organ harvest and processing, and sample storage and shipment• Performed phagocytosis assay to quantitate phagocytosis in mice spleen cells and study the effects of Cytochalasin A in inhibiting phagocytosis• Used BD Accuri C6 cytometer to characterize T, B, and NK cells in mice and rat blood samples• Processed non-human primate and human blood samples for serum collection• Established SOP for assays and laboratory equipment• Validated and calibrated equipment and completed documentation according to SOP

• Studied pulmonary cells to understand their function in initiation and regulation of immune responses• Assisted in developing and troubleshooting methods and protocols for immunophenotyping and trained three postdoctoral fellows, one graduate student, and one undergraduate student in flow cytometry• Analyzed pulmonary tissue samples using flow cytometry and FlowJo to show decreased infiltration of inflammatory monocytes in mice treated with a chemokine receptor 2 (CCR2)-antagonist, and demonstrate that mice injected with CCR2-antagonist showed reduced pulmonary immune pathology and decreased mortality and morbidity to influenza• Initiated a project to characterize CX3C chemokine receptor 1 (CX3CR1)-Cre-GFP transgenic mice and used genotyping and immunophenotyping to identify cell population and cell migration of green fluorescent protein (GFP) positive cells• Developed skills in cell sorting and cell fixation• Managed supply ordering, scheduled laboratory events, and organized and stocked inventory• Calibrated and maintained laboratory equipment

• Shadowed doctors and observed treatment of iris melanoma and secondary neovascular glaucoma using Bevacizumab and plaque radiotherapy• Created database of patient files by cancer types and treatment plans to identify shared associations and treatment responses which resulted in a collaborative publication

• Studied metallo-biochemistry and enzymology in the fields of structural biology, molecular biology, and synthetic chemistry to develop better protein based catalysts for academic and industrial applications• Used protein purification, protein engineering, and enzyme kinetics to create, identity, and characterize mutant proteins with enhanced catalysis and substrate recognition in pyruvate aldolase

• Studied the roles and mechanisms of the tumor suppressor gene BRCA1 in the development and progression of breast cancer• Used DNA/RNA extraction and purification, electrophoresis, polymerase chain reaction (PCR), quantitative PCR, polyacrylamide gel electrophoresis (PAGE), and Western blot to investigate the effects of BRCA1 gene expression levels and cytotoxicity in human breast cancer cells• Developed skills in aseptic techniques, microbial culture, and cell culture