Brian Kang Email & Phone Number

• Design and perform studies to identify lead compound and progress through candidate selection: these activities include performing in vitro studies such as minimum inhibitory concentration (MIC) assay/MIC-lowering.
• Perform activities to support biological studies for NIH contracts, internal research projects, and CRO studies
• Collaborate with CRO to effectively carry out ADME, PK, and toxicology assessments in rodents
• Carry out bioanalytical method development, validation, and sample analysis using LC-MS/MS
• Perform activities to support the chemical synthesis of compounds for Agile Sciences' medicinal chemistry program and other chemistry needs and manage medicinal chemistry databases
• Develop, troubleshoot, and prepare drug formulations

Morrisville, NC

• Project leader for host resistance and immunotoxicology studies that implement standard operating procedures (SOP) and good laboratory practices (GLP)
• Coordinate research technicians and interns and ensure study tasks such as reagent preparation, animal doing, and organ collection are carried out according to SOP and GLP guidelines
• Budget and order animals and reagents necessary for studies and prepare test articles according to study protocol
• Prepare data entry forms and establish templates to facilitate data entry and analysis
• Verify data entry, perform calculations and statistical analyses, and present final data package to senior management in oral or written formats
• Identify potential quality control (QC) or study protocol failures and notify quality assurance officer and study director

Morrisville, NC

• Performed preclinical host resistance and immunotoxicology assessments that implemented standard operating procedures (SOP) and good laboratory practices (GLP)
• Participated in host resistance and infectious disease models via animal receipt and randomization, animal weight documentation, infection, dose (subcutaneous/intraperitoneal/oral gavage/intravenous), euthanization via.
• Performed phagocytosis assay to quantitate phagocytosis in mice spleen cells and study the effects of Cytochalasin A in inhibiting phagocytosis
• Used BD Accuri C6 cytometer to characterize T, B, and NK cells in mice and rat blood samples
• Processed non-human primate and human blood samples for serum collection
• Established SOP for assays and laboratory equipment

• Studied pulmonary cells to understand their function in initiation and regulation of immune responses
• Assisted in developing and troubleshooting methods and protocols for immunophenotyping and trained three postdoctoral fellows, one graduate student, and one undergraduate student in flow cytometry
• Analyzed pulmonary tissue samples using flow cytometry and FlowJo to show decreased infiltration of inflammatory monocytes in mice treated with a chemokine receptor 2 (CCR2)-antagonist, and demonstrate that mice.
• Initiated a project to characterize CX3C chemokine receptor 1 (CX3CR1)-Cre-GFP transgenic mice and used genotyping and immunophenotyping to identify cell population and cell migration of green fluorescent protein (GFP).
• Developed skills in cell sorting and cell fixation
• Managed supply ordering, scheduled laboratory events, and organized and stocked inventory

• Shadowed doctors and observed treatment of iris melanoma and secondary neovascular glaucoma using Bevacizumab and plaque radiotherapy
• Created database of patient files by cancer types and treatment plans to identify shared associations and treatment responses which resulted in a collaborative publication

• Studied metallo-biochemistry and enzymology in the fields of structural biology, molecular biology, and synthetic chemistry to develop better protein based catalysts for academic and industrial applications
• Used protein purification, protein engineering, and enzyme kinetics to create, identity, and characterize mutant proteins with enhanced catalysis and substrate recognition in pyruvate aldolase

• Studied the roles and mechanisms of the tumor suppressor gene BRCA1 in the development and progression of breast cancer
• Used DNA/RNA extraction and purification, electrophoresis, polymerase chain reaction (PCR), quantitative PCR, polyacrylamide gel electrophoresis (PAGE), and Western blot to investigate the effects of BRCA1 gene.
• Developed skills in aseptic techniques, microbial culture, and cell culture