Karin De Cortie Email and Phone Number

Art12 related techniqueso Total Body- and Kidney Irradiation using Pantak Irradiatoro Blood sampling, Urine collection, Autopsy, PerfusionFlowCytometryo Aria Sorter: Macrophage isolation/sortingo FACS Calibur, WBC subtypes and Chimerism determinationsMolecular techniqueso Isolation of RNA (Trizol / Rneasy kit) / cDNA synthesiso Quantitative Real-Time PCR and conventional PCRProtein worko Multiplex assay; Milliplex cytokines / chemokineso Western… Show more Art12 related techniqueso Total Body- and Kidney Irradiation using Pantak Irradiatoro Blood sampling, Urine collection, Autopsy, PerfusionFlowCytometryo Aria Sorter: Macrophage isolation/sortingo FACS Calibur, WBC subtypes and Chimerism determinationsMolecular techniqueso Isolation of RNA (Trizol / Rneasy kit) / cDNA synthesiso Quantitative Real-Time PCR and conventional PCRProtein worko Multiplex assay; Milliplex cytokines / chemokineso Western blotting, Immune precipitationHistological Stainingso Immuno-Fluorescent (IF), Immuno-Histo-Chemical (IHC), Histologicalo Paraffin- and cryo sectionsMicroscopyo Confocal, CCD B/W, CCD ColorCell cultureo Isolation of Bone-Marrow-Derived-Cells (BMDC’s) o Cell isolation using magnetic bead separation (MACS)Othero Determine BUN (Blood-Urea-Nitrogen) and Haematocrit values in blood samples Show less

Art12 related worko Models for rheumatoid arthritiso Intra-articular injections (knee and ankle joint), i.v injectionsMolecular techniqueso Quantitative Real-Time PCR (TaqMan probes)o Cloning experimentsProtein worko ELISA’s (EliSpot) and Western blottingHistologicalo Stainings: Immuno-Histo-Chemical (IHC) stainings and histologicalo Microtome and cryotome sectioningCell cultureo Transduction and transfection experimentso Biological… Show more Art12 related worko Models for rheumatoid arthritiso Intra-articular injections (knee and ankle joint), i.v injectionsMolecular techniqueso Quantitative Real-Time PCR (TaqMan probes)o Cloning experimentsProtein worko ELISA’s (EliSpot) and Western blottingHistologicalo Stainings: Immuno-Histo-Chemical (IHC) stainings and histologicalo Microtome and cryotome sectioningCell cultureo Transduction and transfection experimentso Biological assaysOthero Managerial work and organizing proceedings Show less

See description Arthrogen

o Intra-cerebro ventriculair cannulation and vena jugularis cannulationo Art12 related behavioral tests and techniqueso Experimental design, Literature research, Data processing

2-d and 3-D Cell culture, Cell isolation, Medium tests, Histological analysis